Heat Shock Protein 65-Reactive T Cells Are Involved in the Pathogenesis of Non-Antigenic Dimethyl Dioctadecyl Ammonium Bromide-Induced Arthritis
| Autor: | Kamal D. Moudgil, Hong Ro Kim, Md. Younus Mia, Malarvizhi Durai |
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| Rok vydání: | 2005 |
| Předmět: |
Male
Chaperonins T-Lymphocytes Immunology Arthritis Priming (immunology) Peptide Autoantigens Arthritis Rheumatoid Pathogenesis Epitopes Bacterial Proteins Antigen Heat shock protein Immune Tolerance medicine Animals Humans Immunology and Allergy Collagen Type II Heat-Shock Proteins Autoimmune disease chemistry.chemical_classification Dose-Response Relationship Drug Chaperonin 60 medicine.disease Arthritis Experimental Molecular biology Peptide Fragments Rats Quaternary Ammonium Compounds Disease Models Animal Animals Newborn chemistry Rats Inbred Lew Rheumatoid arthritis Female |
| Zdroj: | The Journal of Immunology. 175:219-227 |
| ISSN: | 1550-6606 0022-1767 |
| Popis: | Dimethyl dioctadecyl ammonium bromide (DDA) (C38H80NBr) is a nonantigenic lipoid material. DDA-induced arthritis (DIA) in the Lewis (LEW) (RT.1l) rat is a new experimental model for human rheumatoid arthritis (RA). DIA is a T cell-mediated autoimmune disease. However, the precise self/foreign Ags associated with the disease process in DIA are not yet known. We observed that LEW rats with DIA spontaneously raised a vigorous T cell response both to 65-kDa self (rat) heat shock protein (Rhsp65) and mycobacterial hsp65 (Bhsp65), but not to another arthritis-related Ag, bovine collagen type II. The T cell response to Rhsp65 was focused predominantly on determinant regions 120–134 and 213–227 of the self protein. Interestingly, pretreatment of adult LEW rats using either a mixture of peptides 120–134 and 213–227 of Rhsp65 or a low nonarthritogenic dose of DDA induced protection against subsequent DIA. Intriguingly, the protection induced by the latter was associated with spontaneous priming of T cells specific for peptide 213–227 of Rhsp65. Similarly, LEW rats neonatally tolerized against either Rhsp65 or Bhsp65 were significantly protected from subsequently induced DIA at adult stage, showing the disease-modulating attribute of the hsp65-specific T cells. Taken together, the above findings demonstrate that the hsp65-directed T cell repertoire is of significance in the pathogenesis of autoimmune arthritis induced by nonantigenic DDA. Like other animal models of RA involving hsp65, these first insights into the disease-associated Ags in the DIA model would pave the way for further understanding of the immunological aspects of induction and regulation of RA. |
| Databáze: | OpenAIRE |
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