Assays for transferrin and transferrin receptors in tumour and other mouse tissues
| Autor: | Sunil De Abrew |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
Male
Lymphoma Cell Population Spleen Transferrin receptor Receptors Cell Surface Biology Iodine Radioisotopes Mice Competitive binding Receptors Transferrin medicine Animals Radiology Nuclear Medicine and imaging education chemistry.chemical_classification education.field_of_study Thymus Neoplasm Transferrin Neoplasms Experimental Thymus Neoplasms medicine.disease Molecular biology medicine.anatomical_structure chemistry Biochemistry Mice Inbred CBA |
| Zdroj: | International journal of nuclear medicine and biology. 8(4) |
| ISSN: | 0047-0740 |
| Popis: | Measurements of the binding of 125 I human transferrin to single cell preparations from mouse tissues indicated that the average number of transferrin receptors per cell was about 10 5 for RILQ lymphoma cells, ∼ 5 × 10 4 for the spleen population, 3 for cells from the thymus, and 6 × 10 4 for mouse reticulocytes. In addition a competitive binding assays was used to estimate, relative to mouse plasma as a standard, the amounts of transferrin in supernatants separated from the first-stage cell suspensions. These measurements indicated that the transferrin contents per g tissue were ∼ 550 μg for tumor, ∼ 300 μg for spleen and thymus. |
| Databáze: | OpenAIRE |
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