Polypeptide Expression in Prostate Hyperplasia and Prostate Adenocarcinoma
Autor: | Ayodele Alaiya, Daniela Volz, Adelaide Carlsson, Gert Auer, Uwe J. Roblick, Sören Huwendiek, Lars Egevad, Stig Linder, Bo Franzén |
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Jazyk: | angličtina |
Rok vydání: | 2000 |
Předmět: |
PCA3
Male Pathology medicine.medical_specialty Prostatic Hyperplasia Adenocarcinoma lcsh:RC254-282 Cytokeratin Prostate medicine Biomarkers Tumor Image Processing Computer-Assisted Humans Electrophoresis Gel Two-Dimensional lcsh:QH573-671 Aged Gel electrophoresis Aged 80 and over Prostate cancer biology lcsh:Cytology Gene Expression Profiling Prostatic Neoplasms Cell Differentiation Middle Aged lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens medicine.disease two‐dimensional gel electrophoresis Proliferating cell nuclear antigen Neoplasm Proteins Gene expression profiling Gene Expression Regulation Neoplastic medicine.anatomical_structure biology.protein Cancer research Other Calreticulin prognostic markers |
Zdroj: | Analytical Cellular Pathology : the Journal of the European Society for Analytical Cellular Pathology Karolinska Institutet Analytical Cellular Pathology, Vol 21, Iss 1, Pp 1-9 (2000) |
ISSN: | 1878-3651 0921-8912 |
Popis: | Cells were collected from prostate hyperplasias (n=6) and prostate carcinomas (n=6) and subjected to two‐dimensional gel electrophoresis (2‐DE). The resulting polypeptide patterns were analysed with the PDQUEST computer software. Malignant tumors showed significant increases in the level of expression of proliferating cell nuclear antigen (PCNA), calreticulin, HSP 90 and pHSP 60, oncoprotein 18(v), elongation factor 2, glutathione‐S‐transferaseπ(GST‐π), superoxide dismutase and triose phosphate isomerase. In addition, decreases in the levels of tropomyosin‐1 and 2 and cytokeratin 18 were observed in prostate carcinomas compared to prostate hyperplasias. This pattern of alterations is similar to that observed in other carcinomas in our previous studies. All malignant tumors showed simultaneous alterations in 5 or more of 9 markers studied, whereas only one case of benign hyperplasia showed alterations in 5 markers. The EST‐data base for prostate tumors available from NCI (CGAP) was searched for the expression of the mRNAs corresponding to proteins identified in our gels. Large differences in the relative expression of mRNAs and proteins were observed. Our data show alterations in the pattern of polypeptide expression in prostate carcinomas which are similar to those observed in other carcinomas. |
Databáze: | OpenAIRE |
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