PATHFAST, a novel chemiluminescent enzyme immunoassay for measuring estradiol in equine whole blood and serum
| Autor: | Kazuyoshi Taya, Kentaro Nagaoka, Yuko Toishi, Nobuo Tsunoda, Gen Watanabe, Kouiti Kume |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
medicine.medical_specialty
Chemiluminescent enzyme immunoassay 040301 veterinary sciences Late gestation Serum estradiol 02 engineering and technology law.invention Immunoenzyme Techniques 0403 veterinary science law Internal medicine Rapid assay medicine Animals Horses Technology Report Whole blood Chemiluminescence chemistry.chemical_classification Estradiol medicine.diagnostic_test 04 agricultural and veterinary sciences Serum concentration 021001 nanoscience & nanotechnology Time-resolved fluoro-immunoassay Enzyme Endocrinology chemistry Immunoassay Luminescent Measurements PATHFAST Animal Science and Zoology 0210 nano-technology hormones hormone substitutes and hormone antagonists |
| Zdroj: | The Journal of Reproduction and Development |
| ISSN: | 1348-4400 0916-8818 |
| Popis: | A novel chemiluminescent enzyme immunoassay system, PATHFAST, for the measurement of estradiol in horses was evaluated. The concentrations of estradiol in the whole blood and serum of mares were measured using PATHFAST and the estradiol concentrations measured by PATHFAST were compared with those measured by a time-resolved fluoro-immunoassay (FIA). To monitor physiological changes, serum estradiol concentrations in mares were measured using PATHFAST throughout the gestation period. The serum estradiol concentrations correlated highly with those in whole blood samples. The serum concentrations of estradiol measured by PATHFAST also correlated well with FIA. Circulating estradiol increased during mid-gestation and high levels of serum estradiol were maintained in late gestation, followed by an abrupt decline to term. These results demonstrate the utility of PATHFAST in equine clinics as an accurate diagnostic tool for the rapid assay of estradiol within 26 min using unextracted whole blood. |
| Databáze: | OpenAIRE |
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