Keratins provide virus-dependent protection or predisposition to injury in coxsackievirus-induced pancreatitis
| Autor: | Diana Toivola, M. Bishr Omary, Arlene Ramsingh, TM Magin, null Ostrowski, Helen Baribault |
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| Rok vydání: | 2009 |
| Předmět: |
chemistry.chemical_classification
0303 health sciences Histology integumentary system biology 030302 biochemistry & molecular biology macromolecular substances Cell Biology Cell Health and Cytoskeleton Coxsackievirus medicine.disease biology.organism_classification Biochemistry Virology Virus 3. Good health 03 medical and health sciences chemistry Structural Biology Keratin medicine Pancreatitis 030304 developmental biology |
| Zdroj: | Cell Health and Cytoskeleton. 1:51-65 |
| ISSN: | 1179-1330 |
| DOI: | 10.2147/chc.s5792 |
| Popis: | DM Toivola1,SE Ostrowski2, H Baribault3, TM Magin4, AI Ramsingh2, MB Omary51Åbo Akademi University, Dept. Biology, BioCity, Turku, Finland and Stanford University School of Medicine and Digestive Disease Center; 2New York State Department of Health, Albany, NY, USA; 3Amgen, South San Francisco, CA, USA; 4University of Bonn, Bonn, Germany; 5Department of Molecular & Integrative Physiology, University of Michigan Medical School, Ann Arbor, Mi, USAAbstract: Keratins 8 and 18 (K8/K18) are the two major intermediate filament proteins in hepatocytes and pancreatic acinar cells. Acinar cell keratins are organized as cytoplasmic and apicolateral filaments. An important role of hepatocyte K8/K18 is to maintain cellular integrity, while this cytoprotective function of K8/K18 is not evident in the pancreas since keratin-deficient mice cope well with pancreatitis models. To further study the roles of keratins in the exocrine pancreas, we used coxsackievirus B4-models, CVB4-V and CVB4-P, to induce severe acute/chronic pancreatitis and acute pancreatitis, respectively, in K8-null (which lack acinar keratins) and K18-null (which lack cytoplasmic keratins) mice. Despite similar virus titers in all mice, CVB4-V resulted in 40% mortality of the K8-null mice 14 days post-infection compared to no lethality of WT and K18-null mice. In contrast, K8-null mice were far less susceptible to CVB4-P-induced damage as determined by histology and serology analysis, and they recover faster than WT and K18-null mice. After CVB4 virus infection, keratins aggregated during acinar degranulation, and K8/K18 site-specific phosphorylation was observed during degranulation and recovery. Hence, keratins significantly affect CVB4 virulence, positively or negatively, depending on the virus subtype and keratin makeup, in a virus replication-independent manner.Keywords: keratin, pancreatitis, coxsackievirus |
| Databáze: | OpenAIRE |
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