Prevalence of antimicrobial resistance and integron gene cassettes in Escherichia coli isolated from yaks (Poephagus grunniens) in Aba Tibetan Autonomous Prefecture, China
| Autor: | Qianru Huang, Hongning Wang, Tianwu An, Jinxin Zeng, Xiaolin Luo, Shengze Xie, Run Cai, Danyu Chen, Xin Yang, Lan Feng, Wencheng Zou, Guangyang Cheng |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
DNA Bacterial China Cefotaxime medicine.drug_class 030106 microbiology Antibiotics Microbial Sensitivity Tests Integron medicine.disease_cause Tibet Microbiology Polymerase Chain Reaction Integrons 03 medical and health sciences Feces Antibiotic resistance Drug Resistance Multiple Bacterial medicine Escherichia coli Animals Humans Escherichia coli Infections biology Escherichia coli Proteins Pathogenic bacteria biochemical phenomena metabolism and nutrition Anti-Bacterial Agents 030104 developmental biology Infectious Diseases Gene cassette Streptomycin Amikacin biology.protein Cattle medicine.drug |
| Zdroj: | Microbial pathogenesis. 111 |
| ISSN: | 1096-1208 |
| Popis: | Background Escherichia coli (E. coli) is one of the most relevant opportunistic pathogenic bacteria as it may cause severe morbidity and mortality in yaks (poephagus grunniens). In recent years, several kinds of antibiotics have been widely used in Tibetan areas to treat the bacterial diseases, resulting in serious repercussions on the bacterial antibiotic resistance in yaks. This investigation was conducted in order to determine the prevalence of antimicrobial resistance and integron gene cassettes in E. coli isolated from yaks in Aba Tibetan Autonomous Prefecture (Aba TAP), China. Methods A total of 278 non-duplicated fresh samples were collected from the yaks in Aba TAP for the isolation and identification of E. coli isolates. Antimicrobial susceptibility testing is performed by using the disc diffusion method according to the Clinical and Laboratory Standards Institute guidelines (CLSI, 2013). Various antibiotic resistance genes and integron gene cassettes were detected by polymerase chain reaction (PCR) and sequencing. Results Overall, a total of 228 E. coli bacteria were isolated from the fresh faeces of yaks in four different geographical regions. 58% of those isolates showed multi-drug resistance capabilities (MDR) in our study. These isolated bacteria showed a high resistance rate to streptomycin (84%), cefotaxime (79%), amikacin (61%) and trimethoprim-sulfamethoxazole (54%). The most common antimicrobial resistance genes in the isolates were bla CTX-M, sul1, aph (3′)-IIa, aac (3)-IIa, aac (6′)-Ib, tetB, with respective detection rates of 65%, 46%, 35%, 13%, 11%, and 10%. Furthermore, 66% and 6% of the strains carried Class 1 and 2 integrons, respectively. However, the class 3 integron was not detected. Gene cassette arrays in the class 1 integron included aadA1, aadA7, aadA5, aadA17, dfrA1, dfrA5, dfrA1-aadA1, dfrA12-aadA2 and dfrA17-aadA5. The most prevalent gene cassette was aadA1 (20%). For the class 2 integron, dfrA1-sat2-aadA1 (6%) and dfrA1-sat1-aadA1 (0.4%) were also detected as part of this research. Conclusion High multi-drug resistance rates have been discovered, as well as a prevalence of antibiotic resistance genes and integron gene cassettes in the E. coli isolated from the faeces of yak. This might create a potential problem for treatment of the yaks' bacterial infections as well as food hygiene for humans. It is therefore urgently necessary to begin continuous surveillance and analysis of antibiotic resistance and integron cassettes in other bacteria from yaks. |
| Databáze: | OpenAIRE |
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