Analysis of the expression of platelet antigens CD9 and CD41/61 in transgenic rabbits with the integrated human blood clotting factor VIII gene construct
| Autor: | Peter Chrenek, Katarina Michalkova, Vladimir Tancin, Jana Jankovičová, M. Simon, Svatoslav Hluchy, L'. Horovská, Jana Antalíková |
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| Rok vydání: | 2011 |
| Předmět: |
Blood Platelets
Platelet Membrane Glycoprotein IIb Physiology Somatic cell Transgene Biophysics Biology Tetraspanin 29 Cell Line Animals Genetically Modified Cell membrane Antigen Antigens CD medicine Animals Humans Platelet Blood Coagulation Gene Clotting factor Factor VIII Membrane Glycoproteins Integrin beta3 General Medicine Molecular biology medicine.anatomical_structure Gene Expression Regulation Cell culture Female Rabbits |
| Zdroj: | General physiology and biophysics. 30:83-87 |
| ISSN: | 1338-4325 |
| DOI: | 10.4149/gpb_2011_si1_83 |
| Popis: | The objective of this research was to study the expression of cell membrane molecules CD9 and CD41/61 of transgenic rabbit with integrated human factor VIII (rhFVIII) gene construct. The expressions of these molecules have been monitored during two lactations of transgenic rabbits and simultaneously compared with the expression of the same molecules of non-transgenic rabbits. The immunochemical analysis by indirect immunofluorescence, ELISA and indirect immunoperoxidase staining of blood cells and udder tissues show that the insertion of the WAP-hFVIII gene construct into the rabbit genome, do not influence the expression of cell membrane antigens CD9 and CD41/61 on the blood platelets, polymorphonuclear blood cells, milk somatic cells and mammary gland tissues. |
| Databáze: | OpenAIRE |
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