Rise in Haemophilus influenzae With Reduced Quinolone Susceptibility and Development of a Simple Screening Method
| Autor: | Shoji Seyama, Takeaki Wajima, Norihisa Noguchi, Takeshi Fujii, Hidemasa Nakaminami, Masanobu Ushio, Yuki Yanagisawa |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Haemophilus Infections medicine.drug_class 030106 microbiology Mutagenesis (molecular biology technique) Microbial Sensitivity Tests Drug resistance Quinolones Biology medicine.disease_cause Polymerase Chain Reaction Microbiology Haemophilus influenzae law.invention 03 medical and health sciences Minimum inhibitory concentration 0302 clinical medicine law Levofloxacin Drug Resistance Bacterial medicine Humans 030212 general & internal medicine Polymerase chain reaction Antimicrobial Quinolone Virology Anti-Bacterial Agents Molecular Typing Infectious Diseases Amino Acid Substitution Pediatrics Perinatology and Child Health medicine.drug |
| Zdroj: | Pediatric Infectious Disease Journal. 36:263-266 |
| ISSN: | 0891-3668 |
| DOI: | 10.1097/inf.0000000000001415 |
| Popis: | BACKGROUND β-Lactamase-nonproducing ampicillin-resistant Haemophilus influenzae are prevalent in Japan. Resistance has increased as a consequence of the expanded use of antimicrobial agents, raising concerns about the rise of multidrug (macrolide and fluoroquinolone)-resistant H. influenzae. METHODS In this study, we investigated susceptibility to fluoroquinolones in H. influenzae clinical isolates from 2013 to 2014 and identified the amino acid substitutions in quinolone resistance-determining regions of gyrA and parC. RESULTS All isolates (n = 145) were susceptible to fluoroquinolones; however, some showed reduced susceptibility. The minimum inhibitory concentration of levofloxacin for these strains was 0.063-0.5 µg/mL, and the strains harbored the amino acid substitution S84L in GyrA. Such strains have seen a significant increase. Importantly, all mutants from 2014 were isolated from pediatric patients. In addition, we developed a simple polymerase chain reaction-based screening method for detecting isolates with reduced fluoroquinolone susceptibility. CONCLUSIONS The mutation in GyrA is important as a first step in the development of fluoroquinolone resistance. Hence, detection of reduced susceptible strains may influence the choice of antimicrobial treatment. |
| Databáze: | OpenAIRE |
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