Evaluation of Matrix-Assisted Laser Desorption Ionization−Time of Flight Mass Spectrometry for Identification of Mycobacterium species, Nocardia species, and Other Aerobic Actinomycetes
| Autor: | B. C. Lucas, Robert C. Walchak, Seanne P. Buckwalter, A. A. Rodning, Sherri L. Wohlfiel, Sharon M. Deml, S. L. Olson, Nancy L. Wengenack, B. J. Connelly |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Microbiology (medical) 030106 microbiology Mass spectrometry Sensitivity and Specificity Nocardia Mycobacterium Microbiology Actinobacteria Mycobacterium tuberculosis 03 medical and health sciences Slowly growing Mycobacteria RNA Ribosomal 16S Humans biology Reproducibility of Results Mycobacteriology and Aerobic Actinomycetes Sequence Analysis DNA biology.organism_classification Bacterial Typing Techniques 030104 developmental biology Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Identification (biology) Bacteria |
| Zdroj: | Journal of Clinical Microbiology. 54:376-384 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.02128-15 |
| Popis: | The value of matrix-assisted laser desorption ionization−time of flight mass spectrometry (MALDI-TOF MS) for the identification of bacteria and yeasts is well documented in the literature. Its utility for the identification of mycobacteria and Nocardia spp. has also been reported in a limited scope. In this work, we report the specificity of MALDI-TOF MS for the identification of 162 Mycobacterium species and subspecies, 53 Nocardia species, and 13 genera (totaling 43 species) of other aerobic actinomycetes using both the MALDI-TOF MS manufacturer's supplied database(s) and a custom database generated in our laboratory. The performance of a simplified processing and extraction procedure was also evaluated, and, similar to the results in an earlier literature report, our viability studies confirmed the ability of this process to inactivate Mycobacterium tuberculosis prior to analysis. Following library construction and the specificity study, the performance of MALDI-TOF MS was directly compared with that of 16S rRNA gene sequencing for the evaluation of 297 mycobacteria isolates, 148 Nocardia species isolates, and 61 other aerobic actinomycetes isolates under routine clinical laboratory working conditions over a 6-month period. MALDI-TOF MS is a valuable tool for the identification of these groups of organisms. Limitations in the databases and in the ability of MALDI-TOF MS to rapidly identify slowly growing mycobacteria are discussed. |
| Databáze: | OpenAIRE |
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