In Toto Imaging of Dynamic Osteoblast Behaviors in Regenerating Skeletal Bone
| Autor: | Ben D. Cox, Kenneth D. Poss, Sumeet Pal Singh, Stefano Di Talia, Alessandro De Simone, Valerie A. Tornini |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Bone Regeneration Cell division Biology Fibroblast growth factor General Biochemistry Genetics and Molecular Biology Article Animals Genetically Modified 03 medical and health sciences Live cell imaging medicine Animals Primordium Bone regeneration Zebrafish Osteoblasts Regeneration (biology) Optical Imaging Osteoblast Cell Differentiation Sciences bio-médicales et agricoles biology.organism_classification Cell biology 030104 developmental biology medicine.anatomical_structure Single-Cell Analysis General Agricultural and Biological Sciences Cell Division |
| Zdroj: | Current biology, 28 (24 |
| Popis: | Summary Osteoblasts are matrix-depositing cells that can divide and heal bone injuries. Their deep-tissue location and the slow progression of bone regeneration challenge attempts to capture osteoblast behaviors in live tissue at high spatiotemporal resolution. Here, we have developed an imaging platform to monitor and quantify individual and collective behaviors of osteoblasts in adult zebrafish scales, skeletal body armor discs that regenerate rapidly after loss. Using a panel of transgenic lines that visualize and manipulate osteoblasts, we find that a founder pool of osteoblasts emerges through de novo differentiation within one day of scale plucking. These osteoblasts undergo division events that are largely uniform in frequency and orientation to establish a primordium. Osteoblast proliferation dynamics diversify across the primordium by two days after injury, with cell divisions focused near, and with orientations parallel to, the scale periphery, occurring coincident with dynamic localization of fgf20a gene expression. In posterior scale regions, cell elongation events initiate in areas soon occupied by mineralized grooves called radii, beginning approximately 2 days post injury, with patterned osteoblast death events accompanying maturation of these radii. By imaging at single-cell resolution, we detail acquisition of spatiotemporally distinct cell division, motility, and death dynamics within a founder osteoblast pool as bone regenerates. |
| Databáze: | OpenAIRE |
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