Capillary zone electrophoresis method for determination of bitter (α- and β-) acids in hop (Humulus lupulus L.) cone extracts
| Autor: | Kęstutis Obelevičius, Audrius Maruška, Žydrūnas Stanius, Olga Kornyšova, Ona Ragažinskienė, Elżbieta Skrzydlewska |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Humulus lupulus
Chromatography biology Plant Extracts Terpenes business.industry Cannabaceae Relative standard deviation Electrophoresis Capillary Reproducibility of Results food and beverages Flowers General Medicine biology.organism_classification Hop (networking) Capillary electrophoresis Species Specificity Cyclohexenes Botany Medicine Spectrophotometry Ultraviolet Research article Humulus business |
| Zdroj: | Advances in Medical Sciences. 54 |
| ISSN: | 1898-4002 1896-1126 |
| Popis: | Purpose: Humulus lupulus (H. lupulus), more commonly known as hop, is a member of the Cannabaceae family with male and female flowers on separate plants. It is native in Europe including Lithuania, Asia and North America. Hop has been recognized as a medicinal plant for centuries, nevertheless different medicinal activities of hop are currently investigated and discovered. An important class of hop compounds is the hop acids, which are classified as a-acids and b-acids. Different varieties of hops vary in amount and composition of hop acids. Methods: Simple capillary zone electrophoresis method has been optimized and applied for the analysis of hop acids in hop cone extracts. Results: With this method the analysis takes ca. 10 min. Repeatability for migration times and peak areas expressed as relative standard deviation were up to 0.21% and 5.96%, respectively. Conclusions: Comparative results of capillary zone electrophoretic analysis of extracts of different hop varieties and conductometric titration, as a standard method for determination of a-acids, are presented. Both methods provide consistent results, however capillary zone electrophoresis is capable of separating co- form of humulones from other forms. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|