Evaluation and validation of two different chromatographic methods (HPLC and LC-MS/MS) for the determination and confirmation of ochratoxin A in pig tissues
| Autor: | Saša Janković, Dragan Milićević, Srđan Stefanović, Slavica Vesković-Moračanin, Verica Jurić |
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| Rok vydání: | 2009 |
| Předmět: |
Ochratoxin A
Spectrometry Mass Electrospray Ionization Swine Electrospray ionization Chemical Fractionation Kidney Mass spectrometry Tandem mass spectrometry Methylation High-performance liquid chromatography chemistry.chemical_compound Blood serum Tandem Mass Spectrometry Animals Ochratoxin Chromatography High Pressure Liquid Chromatography Reproducibility of Results Kidney metabolism General Medicine Ochratoxins Pollution Spectrometry Fluorescence Liver chemistry Food Science |
| Zdroj: | Journal of Environmental Science and Health, Part B. 44:781-787 |
| ISSN: | 1532-4109 0360-1234 |
| DOI: | 10.1080/03601230903238327 |
| Popis: | This study was undertaken to compare two different analytical methods for the determination and confirmation of ochratoxin A (OTA) in blood serum, kidney and liver of pigs. Sample clean-up was based on liquid-liquid phase extraction. The detection of OTA was accomplished with high-performance liquid chromatography (HPLC) combined either with fluorescence detection (FL) or electro spray ionization (ESI+) tandem mass spectrometry (MS-MS). Comparative method evaluation was based on the investigation of 90 samples of blood serum, kidney and liver per animal originating from different regions of Serbia. The analytical results are discussed in view of the respective method validation data and the corresponding experimental protocols. In general, analytical data obtained with (LC-MS-MS) liquid chromatography electro spray tandem mass spectro metry detection offered comparable good results at the sub-ppb concentration level. The results indicate that the liquid chromatography electro spray tandem mass spectrometric (LC-MS/MS) method was more specific and sensitive for the analysis and confirmation of ochratoxin A in pig tissues then high pressure liquid chromatography (HPLC) method after methylation of OTA. |
| Databáze: | OpenAIRE |
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