Bovine antibody-enriched whey to aid in the prevention of a relapse of Clostridium difficile-associated diarrhoea: preclinical and preliminary clinical data
| Autor: | Ed J. Kuijper, Jan W. van ’t Wout, Peter J Veldkamp, Jaap T. van Dissel, Sandra C Numan, Nanda De Groot, Charles Maria Hubert Hensgens |
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| Rok vydání: | 2005 |
| Předmět: |
Microbiology (medical)
Diarrhea medicine.drug_class Antibiotics Pilot Projects Microbiology Cohort Studies Immune system Cricetinae Medicine Animals Humans Prospective Studies Adverse effect Feces biology business.industry Clostridioides difficile General Medicine Clostridium difficile Antibodies Bacterial Immunization Immunology biology.protein Clostridium Infections Cattle Antibody medicine.symptom business |
| Zdroj: | Journal of medical microbiology. 54(Pt 2) |
| ISSN: | 0022-2615 |
| Popis: | In a pilot study, the feasibility of immune whey protein concentrate (40 %; immune WPC-40) to aid the prevention of relapse of Clostridium difficile diarrhoea was evaluated. Immune WPC-40 was made from milk after immunization of Holstein–Frisian cows with C. difficile-inactivated toxins and killed whole-cell C. difficile. Immune WPC-40 contained a high concentration of specific sIgA antibodies, and was effective in neutralizing the cytotoxic effect of C. difficile toxins in cell assays in vitro. Immune WPC-40 conferred protection from otherwise lethal C. difficile-associated caecitis in hamsters. To obtain preliminary data in humans, 16 patients (10 male; median 57 years) with toxin- and culture-confirmed C. difficile diarrhoea were enrolled in an uncontrolled cohort study. Nine had a history of relapsing C. difficile diarrhoea. After completion of standard antibiotic treatment, the patients received immune WPC-40 TID for 2 weeks; it was well tolerated and no treatment-related adverse effects were observed. In all but one case, C. difficile toxins had disappeared from the faeces upon completion of treatment. During a follow-up period of median 333 days (range 35 days to 1 year), none of the patients had suffered another episode of C. difficile diarrhoea. These preliminary data suggest that immune whey protein concentrate-40 may be of help in the prevention of relapse of C. difficile diarrhoea. |
| Databáze: | OpenAIRE |
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