Endothelial Colony-Forming Cells Dysfunctions Are Associated with Arterial Hypertension in a Rat Model of Intrauterine Growth Restriction
| Autor: | Anne Wilson, Françoise Dignat-George, Estelle Guillot, Stéphanie Simoncini, Catherine Yzydorczyk, Anne-Christine Peyter, Umberto Simeoni, Steeve Menétrey, Hanna Coppola, Nathalie Rosenblatt-Velin, Leila Zippo, Romain Bedel, Jean-Baptiste Armengaud, Isaline Bachmann, Florence Sabatier, Angela Rocca |
|---|---|
| Přispěvatelé: | Centre recherche en CardioVasculaire et Nutrition = Center for CardioVascular and Nutrition research (C2VN), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université de Lausanne = University of Lausanne (UNIL), RANCHON, GUILLAUME |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
CD31 medicine.medical_specialty arterial hypertension intrauterine growth restriction Endothelium Angiogenesis Offspring QH301-705.5 [SDV]Life Sciences [q-bio] Intrauterine growth restriction Blood Pressure Stress-induced premature senescence Immunofluorescence Article Catalysis Inorganic Chemistry endothelial colony-forming cells developmental programming Internal medicine medicine Animals oxidative stress Physical and Theoretical Chemistry Biology (General) Molecular Biology QD1-999 Cellular Senescence Spectroscopy Cell Proliferation Fetal Growth Retardation Neovascularization Pathologic medicine.diagnostic_test business.industry Organic Chemistry General Medicine medicine.disease Rats Computer Science Applications [SDV] Life Sciences [q-bio] Chemistry Endocrinology medicine.anatomical_structure Cord blood stress-induced premature senescence Female business |
| Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences, 2021, 22 (18), pp.10159. ⟨10.3390/ijms221810159⟩ Volume 22 Issue 18 International Journal of Molecular Sciences, Vol 22, Iss 10159, p 10159 (2021) International journal of molecular sciences, vol. 22, no. 18, pp. 10159 |
| ISSN: | 1661-6596 1422-0067 |
| Popis: | Infants born after intrauterine growth restriction (IUGR) are at risk of developing arterial hypertension at adulthood. The endothelium plays a major role in the pathogenesis of hypertension. Endothelial colony-forming cells (ECFCs), critical circulating components of the endothelium, are involved in vasculo-and angiogenesis and in endothelium repair. We previously described impaired functionality of ECFCs in cord blood of low-birth-weight newborns. However, whether early ECFC alterations persist thereafter and could be associated with hypertension in individuals born after IUGR remains unknown. A rat model of IUGR was induced by a maternal low-protein diet during gestation versus a control (CTRL) diet. In six-month-old offspring, only IUGR males have increased systolic blood pressure (tail-cuff plethysmography) and microvascular rarefaction (immunofluorescence). ECFCs isolated from bone marrow of IUGR versus CTRL males displayed a decreased proportion of CD31+ versus CD146+ staining on CD45− cells, CD34 expression (flow cytometry, immunofluorescence), reduced proliferation (BrdU incorporation), and an impaired capacity to form capillary-like structures (Matrigel test), associated with an impaired angiogenic profile (immunofluorescence). These dysfunctions were associated with oxidative stress (increased superoxide anion levels (fluorescent dye), decreased superoxide dismutase protein expression, increased DNA damage (immunofluorescence), and stress-induced premature senescence (SIPS increased beta-galactosidase activity, increased p16INK4a, and decreased sirtuin-1 protein expression). This study demonstrated an impaired functionality of ECFCs at adulthood associated with arterial hypertension in individuals born after IUGR. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|