Selective and compartmentalized myelin expression of HspB5
| Autor: | Vincent O'Connor, Victor Hugh Perry, Robert Fern, Andreas Wyttenbach, Nyasha Matinyarare, Shmma Quraishe |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Central Nervous System Aging In situ hybridization 03 medical and health sciences Myelin Mice 0302 clinical medicine medicine Animals RNA Messenger Cytoskeleton Myelin Sheath Messenger RNA biology General Neuroscience RNA Computational Biology Membrane Proteins alpha-Crystallin B Chain Myelin Basic Protein Oligodendrocyte Myelin basic protein Cell biology Mice Inbred C57BL Microscopy Electron 030104 developmental biology medicine.anatomical_structure nervous system Membrane protein Biochemistry biology.protein 2' 3'-Cyclic-Nucleotide Phosphodiesterases 030217 neurology & neurosurgery |
| Zdroj: | Neuroscience. 316 |
| ISSN: | 1873-7544 |
| Popis: | In the present study, we reveal myelin specific expression and targeting of mRNA and biochemical pools of HspB5 in the mouse CNS. Our observations are based on in-situ hybridisation, electron microscopy and co-localisation with 2’,3’-Cyclin-Nucleotide 3’-Phosphodiesterase (CNPase), reinforcing this myelin selective expression. HspB5 mRNA might be targeted to these structures based on its presence in discrete clusters resembling RNA granules and the presence of a putative RNA transport signal. Further, sub-cellular fractionation of myelin membranes reveals a distinct sub-compartment specific association and detergent solubility of HspB5. This is akin to other abundant myelin proteins and is consistent with HspB5’s association with cytoskeletal/membrane assemblies. Oligodendrocytes have a pivotal role in supporting axonal function via generating and segregating the ensheathing myelin. This specialisation places extreme structural and metabolic demands on this glial cell type. Our observations place HspB5 in oligodendrocytes which may require selective and specific chaperone capabilities to maintain normal function and neuronal support. |
| Databáze: | OpenAIRE |
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