Evaluation of serum arginase I as an oxidative stress biomarker in a healthy Japanese population using a newly established ELISA
| Autor: | Keiki Ogino, Noriko Sakano, Masahiko Bando, Yorika Tsukiyama, Heri Setiawan, Masayuki Kubo, Da Hong Wang, Ikuo Murakami, Hidekazu Takahashi, Yasukazu Ohmoto |
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| Rok vydání: | 2013 |
| Předmět: |
Adult
Male Silver Staining medicine.medical_specialty Erythrocytes Urinary system Clinical Biochemistry Enzyme-Linked Immunosorbent Assay Biology medicine.disease_cause Statistics Nonparametric law.invention Asian People Japan law Internal medicine Diabetes mellitus Leukocytes medicine Animals Humans ARG1 Arginase Deoxyguanosine General Medicine Reference Standards medicine.disease Oxidative Stress Endocrinology 8-Hydroxy-2'-Deoxyguanosine Health Immunology Recombinant DNA Biomarker (medicine) Population study Electrophoresis Polyacrylamide Gel Female Rabbits Reagent Kits Diagnostic Biomarkers Oxidative stress |
| Zdroj: | Clinical Biochemistry. 46:1717-1722 |
| ISSN: | 0009-9120 |
| Popis: | Objective We reported previously that serum arginase I increased in asthmatic patients and was associated with oxidative stress in a small healthy population. However, the exact association of arginase I with oxidative stress is not known. The present study aimed to analyze the association of arginase I with oxidative stress in a larger healthy population by a newly established ELISA. Design and methods The new ELISA for the measurement of human arginase I was established by generating recombinant arginase I protein in human arginase I gene-transfected Escherichia coli via an ARG1 cDNA fragment-inserted vector and -specific antibody in rabbits. Serum arginase I was evaluated in a cross-sectional study on a healthy population (n = 721) by comparing a commercial ELISA kit with the new ELISA. Results The mean levels of serum arginase I were 20.3 ± 0.7 ng/mL and 4.7 ± 0.2 ng/mL using the commercial ELISA kit and the new ELISA, respectively. Arginase I was correlated with WBC, RBC, hs-CRP, 8-OHdG, HDL-c, ALT, and BMI. Logistic regression analysis showed independent positive associations of arginase I with WBC, RBC, and urinary 8-OHdG and inverse independent associations with serum insulin and age. The association of arginase I with hs-CRP was not independent. Conclusion The independent associations of arginase I with urinary 8-OHdG and serum insulin may reflect its involvement in oxidative stress and diabetes mellitus. |
| Databáze: | OpenAIRE |
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