Fluorescent labelling of Beet necrotic yellow vein virus and Beet soil-borne mosaic virus for co- and superinfection experiments in Nicotiana benthamiana
| Autor: | Daniela S. Christ, Dietmar Riedel, Edgar Maiss, Mark Varrelmann, Hamza Mohammad, Marlene Laufer, Sebastian Liebe |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
biology Mosaic virus viruses Secondary infection fungi food and beverages Nicotiana benthamiana biology.organism_classification Potato virus X Virology Benyvirus Virus 03 medical and health sciences 030104 developmental biology Tobacco rattle virus Beet necrotic yellow vein virus |
| Zdroj: | Journal of General Virology. 99:1321-1330 |
| ISSN: | 1465-2099 0022-1317 |
| Popis: | Infectious full-length clones of Beet necrotic yellow vein virus (BNYVV) and Beet soil-borne mosaic virus (BSBMV), both genus Benyvirus, were used for fluorescent labelling with the objective to study their interaction in coinfection and superinfection experiments. Fluorescent labelling was achieved by replacing a part of the RNA2 encoded coat protein read-through domain with either GFP or mRFP fluorescent marker proteins. This resulted in a translational fusion comprising the coat and the fluorescent protein. The labelled viruses were infectious and moved systemically in Nicotiana benthamiana, producing wild-type-like symptoms. Virus particles could be observed by electron microscopy, demonstrating that the viral read-through domain is dispensable for particle formation. Coinfection experiments revealed a spatial separation of differentially labelled populations of both identical and different Benyvirus species after N. benthamiana agro-inoculation. Identical observations were obtained when Tobacco rattle virus (TRV) was differentially labelled and used for coinfection. In contrast, coinfections of BSBMV with Potato virus X (PVX) or TRV resulted in many co-infected cells lacking spatial separation. Micro-projectile co-bombardment of N. benthamiana leaves revealed that two differently labelled populations of the same virus co-infected only a few cells before starting to separate. In superinfection experiments with N. benthamiana, BSBMV and BNYVV were unable to establish a secondary infection in plants that were previously infected with BNYVV or BSBMV. Taken together, this is the first work to describe the interaction between two economically important Benyviruses using fluorescence-labelled full-length clones. |
| Databáze: | OpenAIRE |
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