Pulsatile activity of isolated heart muscle cells after freezing storage
| Autor: | E. Schöpf-Ebner, O.M. Bucher, W.O. Gross |
|---|---|
| Rok vydání: | 1968 |
| Předmět: |
Dimethyl sulfoxide
Cell preparation Muscles Myocardium Pulsatile flow Heart General Medicine Isolated heart Anatomy Chick Embryo Biology Chick embryos General Biochemistry Genetics and Molecular Biology Andrology chemistry.chemical_compound chemistry Freezing Myocyte Animals Dimethyl Sulfoxide Tissue Preservation General Agricultural and Biological Sciences |
| Zdroj: | Cryobiology. 4(4) |
| ISSN: | 0011-2240 |
| Popis: | Summary The technique of freeze-storage in which the protective dimethyl sulfoxide never comes in contact with the cells at temperatures above 0°C 3 was applied to suspensions of isolated heart muscle cells. A frozen cell preparation of full biological value for “banking” could not yet be provided, the survival rate being too small (maximal 8%). Nevertheless, several of the frozen heart muscle cells of 8-day chick embryos regained beating activity after thawing (about 5 to 20% of the surviving cells). This proves that the faculty of autonomous pulsation of these isolated cells can, in principle, withstand freezing. |
| Databáze: | OpenAIRE |
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