Antibacterial activity and subchronic toxicity of Cassia fistula L. barks in rats
| Autor: | Tiana Milanda, Muhaimin Muhaimin, Anis Yohana Chaerunisaa, Yasmiwar Susilawati, Anas Subarnas, Rini Hendriani |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Shigella dysenteriae
medicine.drug_class Health Toxicology and Mutagenesis Antibiotics Ethyl acetate 010501 environmental sciences Toxicology medicine.disease_cause 01 natural sciences 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine lcsh:RA1190-1270 In vivo Cassia medicine Subchronic toxicity lcsh:Toxicology. Poisons 0105 earth and related environmental sciences Antibacterial agent ComputingMethodologies_COMPUTERGRAPHICS biology Traditional medicine Chemistry Pathogenic bacteria Regular Article Salmonella typhosa biology.organism_classification Cassia fistula L Antibacterial activity 030217 neurology & neurosurgery |
| Zdroj: | Toxicology Reports Toxicology Reports, Vol 7, Iss, Pp 649-657 (2020) |
| ISSN: | 2214-7500 |
| Popis: | Graphical abstract Highlights • Extract and ethyl acetate fraction of Cassia fistula L. have potent antibacterial activity against resistant bacteria such as Salmonella typhosa. • Extract and Ethyl acetate fraction of Cassia fistula L. barks revealed the most active in vivo antibacterial activity. • At 1000 mg/kg dose of extract exposured for 90 days, histological damages were found either in liver or kidney, but after 30 days the abnormality fully were recovered. Increasing incidence of antibiotic resistance necessitates the development of more potent antibiotics. The aim of this work was to evaluate the antibacterial activity of Cassia fistula L. barks as an alternative agent for resistant pathogenic bacteria. The C. fistula barks were extracted with ethanol, followed by partition of the extract to give n-hexane, ethyl acetate and water fractions. An in vitro antibacterial assay was conducted to evaluate inhibitory activity of the extract and fractions against Salmonella typhosa and Shigella dysenteriae. An in vivo antibacterial activity was examined using S. typhosa-infected mouse models, in which the colony number of S. typhosa were counted from the infected rats’ feces. Assesment on safety of the extract was conducted by a subchronic toxicity test which mainly examined alteration occured in biochemical parameters and hystopatological conditions of livers and kidneys. The results showed that the ethanol extract inhibited the growth of both S. typhosa and S. dysenteriae with the MIC of 0.3125% w/v, and the ethyl acetate fraction with the MIC of 0.625% b/v. In the in vivo antibacterial assay, the extract at three doses decreased the colony number of S. typhosa significantly, and after the fourth to sixth days, the precentage of decrease reached more than 90% by 1000 mg/kg dose. The subchronic toxicity test revealed that after the extract exposured for 90 days, a dose of 1000 mg/kg induced liver and kidney damages histologically, however, it returned to normal condition after 30 days of recovery. The results of this study indicated that the extract of C. fistula L. barks had potent in vivo antibacterial activity against S. typhosa as sample of resistant bacteria, and is safe to be used as a herbal medicine, preferably at a dose lower than 1000 mg/kg. |
| Databáze: | OpenAIRE |
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