Determination of thyreostats in urine and thyroid gland by ultra high performance liquid chromatography tandem mass spectrometry
| Autor: | Bruno Le Bizec, Madis Lõhmus, Kalle Kallaste |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Electrospray
Chromatography Swine Chemistry Organic Chemistry Thyroid Gland General Medicine Reversed-phase chromatography Tandem mass spectrometry Mass spectrometry Biochemistry High-performance liquid chromatography Analytical Chemistry Antithyroid Agents Tandem Mass Spectrometry Liquid chromatography–mass spectrometry Animals media_common.cataloged_instance Cattle Solid phase extraction European union Chromatography High Pressure Liquid media_common |
| Zdroj: | Journal of Chromatography A. 1216:8080-8089 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/j.chroma.2009.04.005 |
| Popis: | Thyreostatic compounds could be illegally administered to animals in order to obtain a weight gain due to a higher retention of water in the edible tissue and the gastro-intestinal tract. In the European Union their use for animal production is banned since 1981. Recently a highly sensitive method exploiting the determination of thyreostats with 3-iodobenzylbromide prior to purification to determine thyreostats in urine and other matrices was reported. For the first time, the UPLC instrumentation was used to separate the 3-iodobenzyl derivatives of various thyreostats. The deuterated internal standards tapazole-d(3) and propylthiouracil-d(5) were for the first time used for the quantification of tapazole, thiouracil, methylthiouracil, propylthiouracil, phenylthiouracil and mercaptobenzimidazole. The confirmative quantitative liquid chromatographic tandem mass spectrometric (LC-MS/MS) method was validated according to Commission Decision 2002/657/EC. The decision limit (CCalpha) and the detection capability (CCbeta) were found to be for all compounds below the recommended value of 10 microg kg(-1). |
| Databáze: | OpenAIRE |
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