IL23R gene variants in relation to IL17A levels and clinical phenotype in patients with ankylosing spondylitis
| Autor: | Johannes C. Nossent, Sylvia Sagen-Johnsen, Gunnstein Bakland |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
musculoskeletal diseases medicine.medical_specialty haplotype phenotype medicine.medical_treatment Consice Report Single-nucleotide polymorphism Gastroenterology 03 medical and health sciences 0302 clinical medicine Rheumatology IL23R Internal medicine Genotype ankylosing spondylitis medicine Allele Genotyping 030203 arthritis & rheumatology business.industry Haplotype 030104 developmental biology Cytokine IL17A business BASFI |
| Zdroj: | Rheumatology Advances in Practice |
| ISSN: | 2514-1775 |
| Popis: | Background IL23 receptor (IL23R) binding by IL23 is required for the maturation of CD4+ cells into Th17 cells and subsequent generation of IL17A and TNF. As IL23R variations contribute to AS susceptibility, we investigated the effect of IL23R variants on cytokine levels and disease measures in an AS cohort. Methods This was a cross-sectional study of AS patients (n = 334, 90% B27+, age 45 years). IL23R genotyping for three non-synonymous single-nucleotide polymorphisms (rs11209026, protective allele A; rs10489629, protective allele A; and rs11209032, risk allele A) was done by Taqman RT-PCR. IL23, IL17A, TNF and IL6 concentrations were determined by sandwich ELISA. Genotypic associations were analysed with non-parametric methods. Results Twenty-two AS patients (6.6%) carried the protective rs11209026 A allele, whereas 206 (61.7%) carried the rs11209032A risk allele (P = 0.03). Two patients homozygous for rs11209026A had late onset, no co-morbidity and undetectable cytokine levels. IL23R genotypes and five common haplotypes were unrelated with age at onset, BASFI or co-morbidity (all P >0.2). There was no overall difference in the concentration of IL17A (184 vs 233 pg/ml, P > 0.2) or IL23 (276 vs 262 pg/ml, P > 0.4) between AS patients and controls, but a global haplotype association (P = 0.01) was observed for IL23 concentrations. Conclusion Homozygosity for rs11209026A is rare in AS patients, but may ameliorate the clinical presentation. IL17A and IL23 levels are similar in controls and AS patients. IL23R variants influence IL23 levels but not IL17A levels in AS patients, suggesting that IL23R impacts more on cell types other than Th17 cells. |
| Databáze: | OpenAIRE |
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