A Microtube Array Membrane (MTAM) Encapsulated Live Fermenting Staphylococcus epidermidis as a Skin Probiotic Patch against Cutibacterium acnes
| Autor: | Chun Ming Huang, Shinta Marito, Albert Jackson Yang, Chee Ho Chew, Chien Chung Chen, John Jackson Yang, Sunita Keshari |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Glycerol C. acnes Antibiotics Chemokine CXCL2 Dermatitis law.invention lcsh:Chemistry 030207 dermatology & venereal diseases chemistry.chemical_compound Probiotic Mice 0302 clinical medicine law Staphylococcus epidermidis fermentation lcsh:QH301-705.5 Spectroscopy Acne Skin biology Chemistry Interleukin General Medicine Computer Science Applications probiotic S. epidermidis medicine.drug_class Catalysis Article Microbiology Inorganic Chemistry 03 medical and health sciences Antibiosis medicine Animals Humans Secretion Physical and Theoretical Chemistry Molecular Biology acne Probiotics Organic Chemistry Propionibacteriaceae biology.organism_classification medicine.disease 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 PSF MTAM Bacteria |
| Zdroj: | International Journal of Molecular Sciences, Vol 20, Iss 1, p 14 (2018) International Journal of Molecular Sciences Volume 20 Issue 1 |
| ISSN: | 1422-0067 |
| Popis: | Antibiotics without selectivity for acne treatment may destroy the beneficial microbes in the human microbiome that helps to fight Cutibacterium acnes (C. acnes), a bacterium associated with inflammatory acne vulgaris. Probiotic treatment by direct application of live Staphylococcus epidermidis (S. epidermidis) onto the open acne lesions may run the risk of bloodstream infections. Here, we fabricated the polysulfone microtube array membranes (PSF MTAM) to encapsulate probiotic S. epidermidis. We demonstrate that the application of the encapsulation of S. epidermidis in PSF MTAM enhanced the glycerol fermentation activities of S. epidermidis. To mimic the granulomatous type of acne inflammatory acne vulgaris, the ears of mice were injected intradermally with C. acnes to induce the secretion of macrophage inflammatory protein-2 (MIP-2), a murine counterpart of human interleukin (IL)-8. The C. acnes-injected mouse ears were covered with a PST MTAM encapsulated with or without S. epidermidis in the presence of glycerol. The application of S. epidermidis-encapsulated PST MTAM plus glycerol onto the C. acnes-injected mouse ears considerably reduced the growth of C. acnes and the production of MIP-2. Furthermore, no S. epidermidis leaked from PSF MTAM into mouse skin. The S. epidermidis-encapsulated PST MTAM functions as a probiotic acne patch. |
| Databáze: | OpenAIRE |
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