Virus isolation, polymerase chain reaction and in vitro antibody production for the diagnosis of pediatric human immunodeficiency virus infection
Autor: | Emmanuel Grimprel, Jean-Claude Nicolas, Fernand Bricout, Nathalie Moniot-Ville, Marie-France Huguet, Antoine Garbarg-Chenon, Anne-Marie Conge, Christian Courpotin, A. Serre, Michel Segondy, Jean-Pierre Vendrell |
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Přispěvatelé: | Université Pierre et Marie Curie - Paris 6 (UPMC), CHU Trousseau [APHP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU) |
Rok vydání: | 1993 |
Předmět: |
Time Factors
HIV Infections Biology HIV Antibodies Peripheral blood mononuclear cell Polymerase Chain Reaction Virus law.invention Acquired immunodeficiency syndrome (AIDS) law Pregnancy Virology Immunopathology medicine Humans Prospective Studies Maternal-Fetal Exchange Polymerase chain reaction Viral culture [CHIM.ORGA]Chemical Sciences/Organic chemistry Infant Newborn Infant Reproducibility of Results medicine.disease Child Preschool Immunology Antibody Formation biology.protein HIV-1 Leukocytes Mononuclear Female Viral disease Antibody Follow-Up Studies |
Zdroj: | Journal of Virological Methods Journal of Virological Methods, Elsevier, 1993, 42 ((1)), pp.117-25 Journal of Virological Methods, 1993, 42 ((1)), pp.117-25 |
ISSN: | 0166-0934 |
Popis: | International audience; Viral culture (VC), polymerase chain reaction (PCR) and in vitro antibody production (IVAP) by peripheral blood mononuclear cells were compared for the early diagnosis of HIV-1 infection in 46 infants born to HIV-1 seropositive mothers. The ten children considered infected on the basis of clinical signs and persistence of anti-HIV-1 antibodies had at least one positive viral culture and seven were always positive in both PCR and IVAP tests. PCR and IVAP tests were occasionally negative in three infected children. Among 30 healthy children who became seronegative and were always negative for viral culture, 22 (73.3%) were also repeatedly negative in PCR and IVAP. We report 6 cases of children classified as P2A at the term of this study but who had lost anti-HIV-1 antibodies. They presented at least one positive viral culture and occasional positive PCR and/or IVAP results. The results indicate that the combination of viral culture, PCR and IVAP tests improves the early diagnosis of pediatric HIV infection. |
Databáze: | OpenAIRE |
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