In vitro evaluation of cross-strain inhibitory effects of IgY polyclonal antibody against H. pylori
| Autor: | Abolfazl Khafri, Morteza Rezaeifard, Roya Solhi, Mahdi Aminian, Masoud Alebouyeh |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Urease Immunoglobulins Enzyme-Linked Immunosorbent Assay Polyethylene glycol Cross Reactions Inhibitory postsynaptic potential Microbiology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Animals Helicobacter pylori biology Strain (chemistry) Antibodies Bacterial Molecular biology In vitro Blot 030104 developmental biology Infectious Diseases chemistry Polyclonal antibodies biology.protein Electrophoresis Polyacrylamide Gel 030211 gastroenterology & hepatology Growth inhibition Chickens |
| Zdroj: | Microbial Pathogenesis. 110:682-687 |
| ISSN: | 0882-4010 |
| DOI: | 10.1016/j.micpath.2017.03.025 |
| Popis: | The present study aimed to evaluate in vitro cross-strain inhibitory effects of IgY polyclonal antibody on both growth and urease enzyme of four local strains of H. pylori . Leghorn chickens were immunized with whole cells of four different strains of H. pylori , separately. Rising of specific IgY was detected by ELISA. The IgY purified using polyethylene glycol method and the purity was evaluated by SDS-PAGE and Western blotting. Each strain was treated with its own-specific and also other strain-specific IgYs. The strain-specific IgY could inhibit the growth of specific strains by 49–72% and also other different strains of H. pylori by 29–86%. Our findings revealed that strain-specific IgY could inhibit urease activity of its own by 64–72% and other different strains by 49–79%. These findings confirmed strain-specific and also cross-strain inhibitory effects of the IgY polyclonal antibody on both growth and urease activity of H. pylori. |
| Databáze: | OpenAIRE |
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