Ecotoxicological risk assessment of wastewater irrigation on soil microorganisms: Fate and impact of wastewater-borne micropollutants in lettuce-soil system

Autor: Laurent Philippot, Nicola Montemurro, Sandra Pérez, Nadine Rouard, Jérémie Béguet, Fabrice Martin-Laurent, Sara Gallego
Přispěvatelé: Agroécologie [Dijon], Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), ENFOCHEM, Environmental Chemistry Department, IDAEA-CSIC, c/Jordi Girona 18-26, 08034 Barcelona, Spain, Montemurro, Nicola, Montemurro, Nicola [0000-0002-7496-203X]
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Irrigation
Agricultural Irrigation
antibiotic resistance
Antibiotic resistance
Health
Toxicology and Mutagenesis
Microorganism
PPCPs
010501 environmental sciences
Wastewater
01 natural sciences
Risk Assessment
ammonium oxidation
Environmental pollution
microbial ecotoxicology
Crop
03 medical and health sciences
Soil
antibiotic degradation
PhACs
media_common.cataloged_instance
GE1-350
European union
Soil Microbiology
030304 developmental biology
0105 earth and related environmental sciences
media_common
0303 health sciences
Ammonium oxidation
biology
Public Health
Environmental and Occupational Health
General Medicine
Lettuce
biology.organism_classification
Pollution
6. Clean water
Environmental sciences
Agronomy
TD172-193.5
13. Climate action
Soil water
[SDE]Environmental Sciences
Environmental science
Antibiotic degradation
Microbial ecotoxicology
Microcosm
Acidobacteria
Zdroj: Ecotoxicology and Environmental Safety
Ecotoxicology and Environmental Safety, Elsevier, 2021, 223, pp.112595. ⟨10.1016/j.ecoenv.2021.112595⟩
Ecotoxicology and Environmental Safety, Vol 223, Iss, Pp 112595-(2021)
Digital.CSIC. Repositorio Institucional del CSIC
instname
ISSN: 0147-6513
1090-2414
Popis: The implementation of the new Water Reuse regulation in the European Union brings to the forefront the need to evaluate the risks of using wastewater for crop irrigation. Here, a two-tier ecotoxicological risk assessment was performed to evaluate the fate of wastewater-borne micropollutants in soil and their ecotoxicological impact on plants and soil microorganisms. To this end, two successive cultivation campaigns of lettuces were irrigated with wastewater (at agronomical dose (not spiked) and spiked with a mixture of 14 pharmaceuticals at 10 and 100 µg/L each) in a controlled greenhouse experiment. Over the two cultivation campaigns, an accumulation of PPCPs was observed in soil microcosms irrigated with wastewater spiked with 100 μg/L of PPCPs with the highest concentrations detected for clarithromycin, hydrochlorothiazide, citalopram, climbazole and carbamazepine. The abundance of bacterial and fungal communities remained stable over the two cultivation campaigns and was not affected by any of the irrigation regimes applied. Similarly, no changes were observed in the abundance of ammonium oxidizing archaea (AOA) and bacteria (AOB), nor in clade A of commamox no matter the cultivation campaign or the irrigation regime considered. Only a slight increase was detected in clade B of commamox bacteria after the second cultivation campaign. Sulfamethoxazole-resistant and -degrading bacteria were not impacted either. The irrigation regimes had only a limited effect on the bacterial evenness. However, in response to wastewater irrigation the structure of soil bacterial community significantly changed the relative abundance of Acidobacteria, Chloroflexi, Verrucomicrobia, Beta-, Gamma- and Deltaprotebacteria. Twenty-eight operational taxonomic units (OTUs) were identified as responsible for the changes observed within the bacterial communities of soils irrigated with wastewater or with water. Interestingly, the relative abundance of these OTUs was similar in soils irrigated with either spiked or non-spiked irrigation solutions. This indicates that under both agronomical and worst-case scenario the mixture of fourteen PPCPs had no effect on soil bacterial community.
This study has been financially supported by the European Union through the WaterJPI-2015 AWARE project (PCIN-2017-067) within the Water Challenges for a Changing World Joint Programming Initiative. The authors thank Damià Barceló, Sandra Pérez and Serge Chiron for having invited us to participate to the AWARE project. The authors also acknowledge SCIEX for loaning the instrument LC/HRMS QTOF X500R. The EU is not liable for any use that may be made of the information contained therein. The authors would also like to thank Marie Christine Breuil for measuring the inorganic nitrogen pools.
Databáze: OpenAIRE
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