Shortening of RNA:DNA Hybrid in the Elongation Complex of RNA Polymerase Is a Prerequisite for Transcription Termination
| Autor: | Natalia Komissarova, Stephanie Solter, Maria L. Kireeva, Mikhail Kashlev, Jodi Becker |
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| Rok vydání: | 2002 |
| Předmět: |
Time Factors
Transcription Genetic Termination factor Molecular Sequence Data RNA-dependent RNA polymerase Small hairpin RNA Potassium Permanganate Transcription (biology) Intrinsic termination Escherichia coli RNA polymerase I Molecular Biology Polymerase Base Sequence Models Genetic biology Nucleic Acid Heteroduplexes Nucleic Acid Hybridization DNA Cell Biology Stem-loop Molecular biology Cell biology biology.protein RNA RNA Polymerase II Protein Binding |
| Zdroj: | Molecular Cell. 10:1151-1162 |
| ISSN: | 1097-2765 |
| Popis: | Passage of E. coli RNA polymerase through an intrinsic transcription terminator, which encodes an RNA hairpin followed by a stretch of uridine residues, results in quick dissociation of the elongation complex. We show that folding of the hairpin disrupts the three upstream base pairs of the 8 bp RNA:DNA hybrid, a major stability determinant in the complex. Shortening the weak rU:dA hybrid from 8 nt to 5 nt causes dissociation of the complex. During termination, the hairpin does not directly compete for base pairing with the 8 bp hybrid. Thus, melting of the hybrid seems to result from spatial restrictions in RNA polymerase that couple the hairpin formation with the disruption of the hybrid immediately downstream from the stem. Our results suggest that a similar mechanism disrupts elongation complexes of yeast RNA polymerase II in vitro. |
| Databáze: | OpenAIRE |
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