Nanobody-driven signaling reveals the core receptor complex in root nodule symbiosis

Autor: Henriette Rübsam, Christina Krönauer, Nikolaj B. Abel, Hongtao Ji, Damiano Lironi, Simon B. Hansen, Marcin Nadzieja, Marie V. Kolte, Dörte Abel, Noor de Jong, Lene H. Madsen, Huijun Liu, Jens Stougaard, Simona Radutoiu, Kasper R. Andersen
Rok vydání: 2023
Předmět:
Zdroj: Rübsam, H, Krönauer, C, Abel, N B, Ji, H, Lironi, D, Hansen, S B, Nadzieja, M, Kolte, M V, Abel, D, de Jong, N, Madsen, L H, Liu, H, Stougaard, J, Radutoiu, S & Andersen, K R 2023, ' Nanobody-driven signaling reveals the core receptor complex in root nodule symbiosis ', Science (New York, N.Y.), vol. 379, no. 6629, pp. 272-277 . https://doi.org/10.1126/science.ade9204
ISSN: 1095-9203
0036-8075
DOI: 10.1126/science.ade9204
Popis: Understanding the composition and activation of multicomponent receptor complexes is a challenge in biology. To address this, we developed a synthetic approach based on nanobodies to drive assembly and activation of cell surface receptors and apply the concept by manipulating receptors that govern plant symbiosis with nitrogen-fixing bacteria. We show that the Lotus japonicus Nod factor receptors NFR1 and NFR5 constitute the core receptor complex initiating the cortical root nodule organogenesis program as well as the epidermal program controlling infection. We find that organogenesis signaling is mediated by the intracellular kinase domains whereas infection requires functional ectodomains. Finally, we identify evolutionarily distant barley receptors that activate root nodule organogenesis, which could enable engineering of biological nitrogen-fixation into cereals.
Databáze: OpenAIRE
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