IP 3 signalling regulates exogenous RNA i in C aenorhabditis elegans
| Autor: | Marie Lopez, María Dolores Sequedo, Anna E Herlihy, Anikó I. Nagy, Rafael P. Vázquez-Manrique, Maxime Bodak, Howard A. Baylis, Christo P. Christov, Roxani Gatsi, Mareike Herzog |
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| Přispěvatelé: | Herzog, Mareike [0000-0001-9747-2327], Baylis, Howard [0000-0002-5959-3256], Apollo - University of Cambridge Repository |
| Rok vydání: | 2015 |
| Předmět: |
inorganic chemicals
calcium signalling Cell signaling Mutant Inositol 1 4 5-Trisphosphate Biology Models Biological Biochemistry RNA interference Image Processing Computer-Assisted Genetics Animals Intestinal Mucosa Caenorhabditis elegans Molecular Biology RNA Double-Stranded Calcium signaling enhanced RNAi Scientific Reports fungi inositol 1 4 5‐trisphosphate RNA biology.organism_classification C. elegans Cell biology Signalling Microscopy Fluorescence Signal transduction Signal Transduction |
| Zdroj: | EMBO reports. 16:341-350 |
| ISSN: | 1469-3178 1469-221X |
| DOI: | 10.15252/embr.201439585 |
| Popis: | RNA interference (RNAi) is a widespread and widely exploited phenomenon. Here, we show that changing inositol 1,4,5-trisphosphate (IP3) signalling alters RNAi sensitivity in Caenorhabditis elegans. Reducing IP3 signalling enhances sensitivity to RNAi in a broad range of genes and tissues. Conversely up-regulating IP3 signalling decreases sensitivity. Tissue-specific rescue experiments suggest IP3 functions in the intestine. We also exploit IP3 signalling mutants to further enhance the sensitivity of RNAi hypersensitive strains. These results demonstrate that conserved cell signalling pathways can modify RNAi responses, implying that RNAi responses may be influenced by an animal's physiology or environment. |
| Databáze: | OpenAIRE |
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