Radiolabelled leucocytes in human pulmonary disease
| Autor: | Neda Farahi, Edwin R. Chilvers, Chrystalla Loutsios, Charlotte Summers, Chandra K. Solanki, Prina Ruparelia, A. Michael Peters, Nicola Tregay, Laurence S C Lok, Daniel Gillett |
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| Přispěvatelé: | Summers, Charlotte [0000-0002-7269-2873], Lok, Laurence [0000-0002-9364-4213], Chilvers, Edwin [0000-0002-4230-9677], Apollo - University of Cambridge Repository |
| Rok vydání: | 2018 |
| Předmět: |
DIFFERENTIAL TRACKING
0301 basic medicine Lung Diseases Pathology RESPIRATORY-DISTRESS-SYNDROME leucocytes 0302 clinical medicine neutrophils Leukocytes In-111 IN-VIVO NEUTROPHIL LIFE-SPAN medicine.diagnostic_test 11 Medical And Health Sciences General Medicine medicine.anatomical_structure Positron emission tomography 030220 oncology & carcinogenesis Isotope Labeling Lobar pneumonia eosinophils Life Sciences & Biomedicine ASTHMA PHENOTYPES medicine.medical_specialty GRANULOCYTE POOL TC-99M-LABELED EOSINOPHILS Granulocyte LOBAR PNEUMONIA lung EOSINOPHILIC INFLAMMATION 03 medical and health sciences Medicine General & Internal General & Internal Medicine medicine Humans Radioisotopes Science & Technology Invited Review Lung Bronchiectasis business.industry Eosinophil medicine.disease Tc-99m RHEUMATOID-ARTHRITIS 030104 developmental biology Positron-Emission Tomography Bone marrow business Ex vivo Granulocytes |
| Popis: | IntroductionRadionuclides for leucocyte kinetic studies have progressed from non-gamma emitting cell-labelling radionuclides through gamma emitting nuclides that allow imaging of leucocyte kinetics, to the next goal of positron emission tomography (PET).Sources of dataMostly the authors’ own studies, following on from studies of the early pioneers.Areas of controversyFrom early imaging studies, it appeared that the majority of the marginated granulocyte pool was located in the lungs. However, later work disputed this by demonstrating the exquisite sensitivity of granulocytes to ex vivo isolation and labelling, and that excessive lung activity is artefactual.Areas of agreementFollowing refinement of labelling techniques, it was shown that the majority of marginated granulocytes are located in the spleen and bone marrow. The majority of leucocytes have a pulmonary vascular transit time only a few seconds longer than erythrocytes. The minority showing slow transit, ~5% in healthy persons, is increased in systemic inflammatory disorders that cause neutrophil priming and loss of deformability. Using a range of imaging techniques, including gamma camera imaging, whole-body counting and single photon-emission computerized tomography, labelled granulocytes were subsequently used to image pulmonary trafficking in lobar pneumonia, bronchiectasis, chronic obstructive pulmonary disease and adult respiratory distress syndrome.Growing pointsMore recently, eosinophils have been separated in pure form using magnetic bead technology for the study of eosinophil trafficking in asthma.Areas timely for developing researchThese include advancement of eosinophil imaging, development of monocyte labelling, development of cell labelling with PET tracers and the tracking of lymphocytes. |
| Databáze: | OpenAIRE |
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