Molecular cloning of SLAP-130, an SLP-76-associated substrate of the T cell antigen receptor-stimulated protein tyrosine kinases
| Autor: | Michael Paskind, Christoph W. Turck, Joanne Kamens, David G. Motto, M A Musci, L. Ranee Hendricks-Taylor, Gary A. Koretzky |
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| Rok vydání: | 1997 |
| Předmět: |
Male
Transcriptional Activation genetic structures T cell Recombinant Fusion Proteins T-Lymphocytes Molecular Sequence Data Receptors Antigen T-Cell Protein tyrosine phosphatase Biology Transfection Biochemistry Jurkat cells Polymerase Chain Reaction Jurkat Cells medicine Cytotoxic T cell Humans IL-2 receptor Amino Acid Sequence RNA Messenger Cloning Molecular Antigen-presenting cell Molecular Biology Adaptor Proteins Signal Transducing Base Sequence NFATC Transcription Factors ZAP70 CD28 Nuclear Proteins Cell Biology Protein-Tyrosine Kinases equipment and supplies Phosphoproteins Molecular biology eye diseases Cell biology DNA-Binding Proteins Molecular Weight medicine.anatomical_structure Organ Specificity Female sense organs Carrier Proteins Transcription Factors |
| Zdroj: | The Journal of biological chemistry. 272(18) |
| ISSN: | 0021-9258 |
| Popis: | Previous work has demonstrated that SLP-76, a Grb2-associated tyrosine-phosphorylated protein, augments Interleukin-2 promoter activity when overexpressed in the Jurkat T cell line. This activity requires regions of SLP-76 that mediate protein-protein interactions with other molecules in T cells, suggesting that SLP-76-associated proteins also function to regulate signal transduction. Here we describe the molecular cloning of SLAP-130, a SLP-76-associated phosphoprotein of 130 kDa. We demonstrate that SLAP-130 is hematopoietic cell-specific and associates with the SH2 domain of SLP-76. Additionally, we show that SLAP-130 is a substrate of the T cell antigen receptor-induced protein tyrosine kinases. Interestingly, we find that in contrast to SLP-76, overexpression of SLAP-130 diminishes T cell antigen receptor-induced activation of the interleukin-2 promoter in Jurkat T cells and interferes with the augmentation of interleukin-2 promoter activity seen when SLP-76 is overexpressed in these cells. These data suggest that SLP-76 recruits a negative regulator, SLAP-130, as well as positive regulators of signal transduction in T cells. |
| Databáze: | OpenAIRE |
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