Immunohistochemistry of cytokeratin proteins in squamous and transitional cell lesions of the urinary tract
| Autor: | M F Tungekar, M S Al-Adnani, K C Gatter |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Pathology
medicine.medical_specialty Biology Epithelium Pathology and Forensic Medicine Schistosomiasis haematobia Cytokeratin Keratin medicine Humans Urothelium chemistry.chemical_classification Carcinoma Transitional Cell Carcinoma in situ General Medicine medicine.disease Squamous metaplasia Staining Molecular Weight medicine.anatomical_structure Urinary Bladder Neoplasms chemistry Lymphatic Metastasis Carcinoma Squamous Cell Keratins Immunohistochemistry Research Article |
| Zdroj: | Journal of Clinical Pathology. 41:1288-1296 |
| ISSN: | 0021-9746 |
| DOI: | 10.1136/jcp.41.12.1288 |
| Popis: | Expression of low and high molecular weight cytokeratin proteins was investigated immunohistochemically in a variety of transitional and squamous epithelial lesions of the urinary tract with and without schistosomiasis. The monoclonal antibodies used were CAM 5.2 and NCL5D3 for low, PK 63 and 121 for high, and MAK 6 for a broad range of intermediate molecular weight cytokeratins. On staining with CAM 5.2 and NCL5D3, urothelial hyperplasias (n = 12) and grades 1 (n = 5) and 2 (n = 10) papillary transitional cell carcinomas showed labelling patterns quite distinct from carcinoma in situ (n = 4) and non-papillary grades 2 (n = 6) and 3 tumours (n = 3). Among squamous lesions only focal positivity was obtained in 14 of 22 moderate to poorly differentiated squamous cell carcinomas. By contrast, PK 63 and 121 stained squamous lesions exclusively. MAK 6 stained the whole range of urothelial and squamous lesions with the exception of squamous metaplasias. Polyclonal antikeratin adequately labelled spindle cell areas of high grade tumours. The distinctive staining patterns given by these or similar antibodies may help in the identification of squamous metaplasia and in diagnosing tumours of the urothelium. |
| Databáze: | OpenAIRE |
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