Effects of rhBMP-2 on Bone Formation Capacity of Rat Dental Stem/Progenitor Cells from Dental Follicle and Alveolar Bone Marrow
| Autor: | Chuan-Jie Li, Lingling E, Rui Xiao, Rong Zhang, Hongchen Liu, Xiao-Cao Ma, Shuo Zhang |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Polyesters Cementoblast Osteocalcin Bone Morphogenetic Protein 2 Gene Expression Biology Rats Sprague-Dawley Extracellular matrix 03 medical and health sciences 0302 clinical medicine stomatognathic system Osteogenesis Transforming Growth Factor beta medicine Animals Humans Progenitor cell Cells Cultured Dental follicle Stem Cells Mesenchymal stem cell Hematopoietic stem cell Cell Differentiation Dental Sac Mesenchymal Stem Cells Osteoblast Cell Biology Hematology Immunohistochemistry Embryonic stem cell Recombinant Proteins Cell biology Durapatite 030104 developmental biology medicine.anatomical_structure Microscopy Electron Scanning Osteopontin Collagen 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Stem Cells and Development. 30:441-457 |
| ISSN: | 1557-8534 1547-3287 |
| DOI: | 10.1089/scd.2020.0170 |
| Popis: | Dental stem/progenitor cells are a promising cell sources for alveolar bone (AB) regeneration because of their same embryonic origin and superior osteogenic potential. However, their molecular processes during osteogenic differentiation remain unclear. The objective of this study was to identify the responsiveness of dental follicle cells (DFCs) and AB marrow-derived mesenchymal stem cells (ABM-MSCs) to recombinant human bone morphogenetic protein-2 (rhBMP-2). These cells expressed vimentin and MSC markers and did not express cytokeratin and hematopoietic stem cell markers and showed multilineage differentiation potential under specific culture conditions. DFCs exhibited higher proliferation and colony-forming unit-fibroblast efficiency than ABM-MSCs; rhBMP-2 induced DFCs to differentiate toward a cementoblast/osteoblast phenotype and ABM-MSCs to differentiate only toward a osteoblast phenotype; and rhBMP-2-induced DFCs exhibited higher osteogenic differentiation potential than ABM-MSCs. These cells adhered, grew, and produced extracellular matrix on nanohydroxyapatite/collagen/poly(l-lactide) (nHAC/PLA). During a 14-day culture on nHAC/PLA, the extracellular alkaline phosphatase (ALP) activity of DFCs decreased gradually and that of ABM-MSCs increased gradually; rhBMP-2 enhanced their extracellular ALP activity, intracellular osteocalcin (OCN), and osteopontin (OPN) protein expression; and DFCs exhibited higher extracellular ALP activity and intracellular OCN protein expression than ABM-MSCs. When implanted subcutaneously in severe combined immunodeficient mice for 3 months, DFCs+nHAC/PLA+rhBMP-2 obtained higher percentage of bone formation area, OCN, and cementum attachment protein expression and lower OPN expression than ABM-MSCs+nHAC/PLA+rhBMP-2. These results showed that DFCs possessed superior proliferation and osteogenic differentiation potential in vitro, and formed higher quantity and quality bones in vivo. It suggested that DFCs might exhibit a more sensitive responsiveness to rhBMP-2, so that DFCs enter a relatively mature stage of osteogenic differentiation earlier than ABM-MSCs after rhBMP-2 induction. The findings imply that these dental stem/progenitor cells are alternative sources for AB engineering in regenerative medicine, and developing dental tissue may provide better source for stem/progenitor cells. |
| Databáze: | OpenAIRE |
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