All-Trans Retinoic Acid Fosters the Multifarious U87MG Cell Line as a Model of Glioblastoma
Autor: | Viera Kútna, Michael Vácha, Zdeňka Polívková, Michael Hudec, Jan Pala, Iva Juříčková, Valerie B. O’Leary, Saak V. Ovsepian, Marie Cerna, Markéta Pokorná |
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Rok vydání: | 2021 |
Předmět: |
medicine.medical_treatment
Retinoic acid Neurosciences. Biological psychiatry. Neuropsychiatry Biology Q1 Stem cell marker Article 03 medical and health sciences chemistry.chemical_compound lncRNA 0302 clinical medicine Prominin-1 medicine Neoplasm chromosome ATRA 030304 developmental biology brain cancer 0303 health sciences Chemotherapy General Neuroscience medicine.disease Phenotype chemistry Cell culture 030220 oncology & carcinogenesis Cancer research CD54 prominin-1 GAS5 RC321-571 |
Zdroj: | Brain Sciences, Vol 11, Iss 812, p 812 (2021) Brain Sciences Volume 11 Issue 6 |
ISSN: | 2076-3425 |
DOI: | 10.3390/brainsci11060812 |
Popis: | Glioblastoma multiforme (GBM) is a primary brain cancer of poor prognosis, with existing treatments remaining essentially palliative. Current GBM therapy fails due to rapid reappearance of the heterogeneous neoplasm, with models suggesting that the recurrent growth is from treatment-resistant glioblastoma stem-like cells (GSCs). Whether GSCs depend on survival/proliferative cues from their surrounding microenvironmental niche, particularly surrounding the leading edge after treatment remains unknown. Simulating human GBM in the laboratory relies on representative cell lines and xenograft models for translational medicine. Due to U87MG source discrepancy and differential proliferation responses to retinoic acid treatment, this study highlights the challenges faced by laboratory scientists working with this representative GBM cell line. Investigating the response to all trans-retinoic acid (ATRA) revealed its sequestering of the prominin-1 stem cell marker. ICAM-1 universally present throughout U87MG was enhanced by ATRA, of interest for chemotherapy targeting studies. ATRA triggered diverse expression patterns of long non-coding RNAs PARTICLE and GAS5 in the leading edge and established monolayer growth zone microenvironment. Karyotyping confirmed the female origin of U87MG sourced from Europe. Passaging U87MG revealed the presence of chromosomal anomalies reflective of structural genomic alterations in this glioblastoma cell line. All evidence considered, this study exposes further phenotypic nuances of U87MG which may belie researchers seeking data contributing towards the elusive cure for GBM. |
Databáze: | OpenAIRE |
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