Synergistic Effect of Two Nanotechnologies Enhances the Protective Capacity of the Theileria parva Sporozoite p67C Antigen in Cattle
| Autor: | Thomas Njoroge, Timothy J. Mahony, Stephen Mwalimu, Neena Mitter, Lucilla Steinaa, James Nyagwange, Karishma T. Mody, Vishvanath Nene, Robert Muriuki, Elias Awino, Chengzhong Yu, Anna Lacasta, Antonino S. Cavallaro, Nicholas N. Ndiwa, Elisabeth Jane Poole, Rosemary Saya, Ine De Goeyse, Jun Zhang, Bing Zhang |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
CD4-Positive T-Lymphocytes
Protozoan Vaccines Hepatitis B virus Immunogen medicine.medical_treatment Theileria parva viruses Immunology Protozoan Proteins Antibodies Protozoan Biology complex mixtures 03 medical and health sciences Mice 0302 clinical medicine Ticks Antigen hemic and lymphatic diseases medicine Immunology and Allergy East Coast fever Animals Mineral Oil Nanotechnology Immunotherapy and Vaccines Viral Core Proteins Vaccination biology.organism_classification Vaccine efficacy Silicon Dioxide Virology Theileriasis RAW 264.7 Cells Tick-Borne Diseases Vaccines Subunit biology.protein Nanoparticles Cattle Antibody Adjuvant 030215 immunology |
| Zdroj: | The Journal of Immunology Author Choice |
| ISSN: | 1550-6606 0022-1767 |
| Popis: | Key Points Multimerization of p67C Ag as nanoparticle increases its immunogenicity. Vaccine efficacy of p67C Ag increases delivered as nanoparticles. Visual Abstract East Coast fever (ECF), caused by Theileria parva, is the most important tick-borne disease of cattle in sub-Saharan Africa. Practical disadvantages associated with the currently used live-parasite vaccine could be overcome by subunit vaccines. An 80-aa polypeptide derived from the C-terminal portion of p67, a sporozoite surface Ag and target of neutralizing Abs, was the focus of the efforts on subunit vaccines against ECF and subjected to several vaccine trials with very promising results. However, the vaccination regimen was far from optimized, involving three inoculations of 450 μg of soluble p67C (s-p67C) Ag formulated in the Seppic adjuvant Montanide ISA 206 VG. Hence, an improved formulation of this polypeptide Ag is needed. In this study, we report on two nanotechnologies that enhance the bovine immune responses to p67C. Individually, HBcAg-p67C (chimeric hepatitis B core Ag virus-like particles displaying p67C) and silica vesicle (SV)–p67C (s-p67C adsorbed to SV-140-C18, octadecyl-modified SVs) adjuvanted with ISA 206 VG primed strong Ab and T cell responses to p67C in cattle, respectively. Coimmunization of cattle (Bos taurus) with HBcAg-p67C and SV-p67C resulted in stimulation of both high Ab titers and CD4 T cell response to p67C, leading to the highest subunit vaccine efficacy we have achieved to date with the p67C immunogen. These results offer the much-needed research depth on the innovative platforms for developing effective novel protein-based bovine vaccines to further the advancement. |
| Databáze: | OpenAIRE |
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