Comparative analysis of post-translational modifications in plasma proteome of patients with cerebral ischemia based on HPLC-MS/MS method
| Autor: | O B Larina, N. A. Petushkova, N B Teryaeva, N. F. Samenkova, D Yu Usachev, Y S Kisrieva, V. G. Zgoda, G P Kuznetsova, I. I. Karuzina |
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| Rok vydání: | 2019 |
| Předmět: |
Proteomics
0301 basic medicine endocrine system Proteome Chemistry Lysine General Medicine General Biochemistry Genetics and Molecular Biology Brain Ischemia Glutamine 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Biochemistry Tandem Mass Spectrometry Acetylation Blood plasma Humans Phosphorylation Threonine Deamidation Protein Processing Post-Translational Chromatography High Pressure Liquid 030217 neurology & neurosurgery |
| Zdroj: | Biomeditsinskaya Khimiya. 65:251-258 |
| ISSN: | 2310-6905 2310-6972 |
| DOI: | 10.18097/pbmc20196503251 |
| Popis: | The relative differences between post-translational modifications (PTM) of proteins in blood plasma samples of patients with cerebral ischemia (CI) and healthy people were investigated using of the method of label-free comparative proteomic analysis based on the technology of tandem HPLC-MS/MS. For PTM detection we used multiple MS/MS search in the database Mascot for variable PTM and Progenesis LS-MS software. In the CI plasma samples, we observed an increase in the proportion of peptides with such PTM as phosphorylation of serine, threonine, and tyrosine, acetylation of lysine and protein N-term, ubiquitination of lysine and deamidation of glutamine related to clinically significant processes were revealed.Issledovany otnositel'nye razlichiia posttransliatsionnykh modifikatsiĭ (PTM) belkov v obraztsakh plazmy krovi bol'nykh tserebral'noĭ ishemieĭ (TsI) i zdorovykh liudeĭ na osnove metoda sravnitel'nogo bezmetkovogo proteomnogo analiza na baze tekhnologii tandemnoĭ VÉZhKh-MS/MS. Dlia obnaruzheniia PTM byl vypolnen mnozhestvennyĭ poisk MS/MS v baze dannykh Mascot s ustanovlennoĭ peremennoĭ PTM s ispol'zovaniem programmnogo obespecheniia Progenesis LS-MS. V plazme bol'nykh TsI nabliudali uvelichenie doli posttransliatsionno-modifitsirovannykh peptidov. Vyiavleny takie PTM belkov, kak fosforilirovanie ostatkov serina, treonina i tirozina, atsetilirovanie ostatkov lizina i N-kontsevoe atsetilirovanie, ubikvitinirovanie ostatkov lizina i dezamidirovanie ostatkov glutamina i asparagina, imeiushchie otnoshenie k klinicheski znachimym protsessam. |
| Databáze: | OpenAIRE |
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