Influence of Salbutamol and Isoproterenol on the Production of TNF and Reactive Oxygen Species by Bovine Alveolar Macrophs and Calcitriol Differentiated HL-60 Cells
| Autor: | Arnulf Seidel, Yiping Gu |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
medicine.medical_specialty
Calcitriol Immunology HL-60 Cells Toxicology chemistry.chemical_compound Internal medicine Isoprenaline Macrophages Alveolar Tumor Cells Cultured medicine Animals Humans Immunology and Allergy Macrophage Albuterol Pharmacology chemistry.chemical_classification Reactive oxygen species Tumor Necrosis Factor-alpha Superoxide Zymosan Isoproterenol Cell Differentiation General Medicine medicine.anatomical_structure Endocrinology chemistry Cattle Tumor necrosis factor alpha Pulmonary alveolus Reactive Oxygen Species medicine.drug |
| Zdroj: | Immunopharmacology and Immunotoxicology. 18:115-128 |
| ISSN: | 1532-2513 0892-3973 |
| DOI: | 10.3109/08923979609007114 |
| Popis: | The influence of the beta-adrenergic agonists Salbutamol and Isoproterenol on the release of reactive oxygen species and Tumor Necrosis Factor (TNF) was tested with bovine alveolar macrophages and HL-60 cells differentiated to macrophages by calcitriol. The production of reactive oxygen species was analyzed by a microplate assay using dichlorofluorescein-diacetate. It could be shown that this method almost exclusively measures superoxide anions. TNF was determined by a bioassay with WEHI cells. The superoxide anion production was stimulated by Zymosan, the TNF release by LPS. By incubation with 5 x 10(-6) and 5 x 10(-7) M Salbutamol or 5 x 10(-7) and 5 x 10(-8) M Isoproterenol prior to the stimulation, the production of superoxide anions as well as of TNF was inhibited to a significant degree. The inhibitory effects of the adrenergic agonists were completely or at least partially inhibited by the respective antagonists, ICI 118.551 and Propranolol, respectively. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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