Polyurethane-based scaffolds for myocardial tissue engineering
| Autor: | Antonella Silvestri, Clotilde Castaldo, Daria Nurzynska, Susanna Sartori, Gianluca Ciardelli, Sara Maria Giannitelli, Pamela Mozetic, Valeria Chiono, Stefania Montagnani, Franca Di Meglio, Emilia Gioffredi, Marcella Trombetta, Monica Boffito, Rita Miraglia, Alberto Rainer |
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| Přispěvatelé: | Chiono, V, Mozetic, P, Boffito, M, Sartori, S, Gioffredi, E, Silvestri, A, Rainer, A, Giannitelli, Sm, Trombetta, M, Nurzynska, DARIA ANNA, DI MEGLIO, Franca, Castaldo, Clotilde, Miraglia, Rita, Montagnani, Stefania, Ciardelli, G. |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Scaffold
Materials science Diol Biomedical Engineering Biophysics Bioengineering Articles Adhesion Bioinformatics Biochemistry Isothermal process Biomaterials Thermogravimetry chemistry.chemical_compound Differential scanning calorimetry Chemical engineering chemistry Surface modification Biotechnology Polyurethane |
| Popis: | Bi-layered scaffolds with a 0°/90° lay-down pattern were prepared by melt-extrusion additive manufacturing (AM) using a poly(ester urethane) (PU) synthesized from poly(ε-caprolactone) diol, 1,4-butandiisocyanate and l -lysine ethyl ester dihydrochloride chain extender. Rheological analysis and differential scanning calorimetry of the starting material showed that compression moulded PU films were in the molten state at a higher temperature than 155°C. The AM processing temperature was set at 155°C after verifying the absence of PU thermal degradation phenomena by isothermal thermogravimetry analysis and rheological characterization performed at 165°C. Scaffolds highly reproduced computer-aided design geometry and showed an elastomeric-like behaviour which is promising for applications in myocardial regeneration. PU scaffolds supported the adhesion and spreading of human cardiac progenitor cells (CPCs), whereas they did not stimulate CPC proliferation after 1–14 days culture time. In the future, scaffold surface functionalization with bioactive peptides/proteins will be performed to specifically guide CPC behaviour. |
| Databáze: | OpenAIRE |
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