Molecular genetic analysis of the candidate gene for MOD, a locus required for self-incompatibility in Brassica rapa
| Autor: | Mikhail E. Nasrallah, Fukai E, Takeshi Nishio |
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| Rok vydání: | 2001 |
| Předmět: |
Candidate gene
DNA Mutational Analysis Restriction Mapping Mutant Locus (genetics) Brassica Biology Aquaporins Ion Channels Transformation Genetic Brassica rapa Genetics RNA Messenger Allele Molecular Biology Gene Peptide sequence Alleles Plant Proteins Models Genetic Homozygote Nucleic acid sequence Exons Sequence Analysis DNA General Medicine Oligonucleotides Antisense Blotting Northern Plants Genetically Modified Blotting Southern Gamma Rays Mutation |
| Zdroj: | Molecular Genetics and Genomics. 265:519-525 |
| ISSN: | 1617-4623 1617-4615 |
| DOI: | 10.1007/s004380000440 |
| Popis: | The MIP-MOD (for MOD-locus associated Major Intrinsic Protein) gene encodes an aquaporin-like product, and has been reported to be a candidate for the MOD gene which is required for the self-incompatibility response in Brassica rapa. In an antisense suppression experiment designed to investigate the role of MIP-MOD, we found that levels of MIP-MOD mRNA in the stigmas of fourteen antisense transgenics, as well as in the self-incompatible cultivar Osome (Osm), were much lower than in the stigmas of the self-incompatible S8 homozygous (S8) strain. Therefore, we analyzed the molecular structure of the MIP-MOD gene in three B. rapa strains: S8, Osm, and the self-compatible var. Yellow Sarson (YS). Nucleotide sequence analysis of the MIP-MOD genes isolated from the three strains revealed that all three encode the same amino acid sequence and that YS and Osm contain the same MIP-MOD allele, designated MIP-MOD(YS). Analysis of other self-incompatible B. rapa strains that are homozygous for the MIP-MOD(YS) allele indicated that high levels of MIP-MOD transcripts are not essential for the self-incompatibility response. Furthermore, a MOD mutant generated by gamma-irradiation was found to contain a wild-type MIP-MOD gene that is expressed at normal levels. These data suggest that MIP-MOD is not MOD itself. We suggest that this gene should be renamed MLM (for MIP gene linked to MOD). |
| Databáze: | OpenAIRE |
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