Enhancer of zeste homolog 2 enhances the migration and chemotaxis of dental mesenchymal stem cells
| Autor: | Yangyang Cao, Haoqing Yang, Shaoyu Duan, Lihua Ge, Runtao Gao, Fei Yan, Huarui Ma |
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| Rok vydání: | 2019 |
| Předmět: |
Medicine (General)
enhancer of zeste homolog 2 macromolecular substances 030204 cardiovascular system & hematology migration Biochemistry Pre-Clinical Research Report human dental tissue-derived mesenchymal stem cells 03 medical and health sciences R5-920 0302 clinical medicine Cell Movement Humans Medicine Enhancer of Zeste Homolog 2 Protein C-X-C motif chemokine Dental Papilla Cells Cultured business.industry Chemotaxis Biochemistry (medical) EZH2 Mesenchymal stem cell Mesenchymal Stem Cells Cell Biology General Medicine Cell biology 030220 oncology & carcinogenesis homing business Homing (hematopoietic) |
| Zdroj: | The Journal of International Medical Research Journal of International Medical Research, Vol 48 (2020) |
| ISSN: | 1473-2300 0300-0605 |
| DOI: | 10.1177/0300060519882149 |
| Popis: | Objective To investigate the function of enhancer of zeste homolog 2 (EZH2) in the migration and chemotaxis of human dental tissue-derived mesenchymal stem cells. Methods The expression of EZH2, C-X-C motif chemokine ligand 11 (CXCL11), CXCL16, and CXCR1 in stem cells from the apical papilla (SCAPs) was determined by real-time reverse transcription PCR and western blotting. The effects of EZH2 on the homing of SCAPs and the effects of EZH2-overexpressing SCAP culture supernatant on periodontal ligament stem cells (PDLSCs) were tested by scratch migration assays and transwell chemotaxis assays. Results EZH2 overexpression significantly enhanced the migration and chemotaxis of SCAPs and upregulated the expression of CXCL11, CXCL16, and CXCR1 in SCAPs. EZH2 depletion had the opposite effect, impairing the migration and chemotaxis of SCAPs and downregulating the expression of CXCL11, CXCL16, and CXCR1. The culture supernatant of EZH2-overexpressing SCAPs advanced the migration and chemotaxis of PDLSCs. Conclusions EZH2 evidently promoted the migration and chemotaxis of SCAPs by upregulating the expression of CXCL11, CXCL16, and CXCR1. Moreover, EZH2-overexpressing SCAPs enhanced the homing, migration, and chemotaxis of PDLSCs via paracrine signaling. |
| Databáze: | OpenAIRE |
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