Dissection of the cAMP induced cytosolic calcium response in Dictyostelium discoideum: the role of cAMP receptor subtypes and G protein subunits
| Autor: | Annette Aichem, Jürgen Sonnemann, Christina Schlatterer |
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| Rok vydání: | 1998 |
| Předmět: |
Time Factors
G protein Protein subunit Biophysics Stimulation Development Signal transduction Biochemistry Dictyostelium discoideum Receptors Cyclic AMP Gβ Cytosol Structural Biology GTP-Binding Proteins Genetics Cyclic AMP Animals Homeostasis Dictyostelium Gα2 Fura2-dextran [Ca2+]i imaging Receptor Molecular Biology Calcium metabolism biology Cell Biology biology.organism_classification Molecular biology Cell biology Kinetics Cell culture Calcium |
| Zdroj: | FEBS letters. 436(2) |
| ISSN: | 0014-5793 |
| Popis: | The cAMP signaling cascade leading to changes in [Ca2+]i in Dictyostelium discoideum was analyzed using cell lines overexpressing single cAMP receptor subtypes (cAR1-cAR3) or lacking the G(alpha2) or G(beta) subunit of the G protein. Imaging of fura2-dextran-loaded amoebae revealed cAMP-induced [Ca2+]i changes characteristic for each receptor subtype activated. Cells expressing distinct subtypes sort to defined zones during multicellular development suggesting involvement of the specific [Ca2+]i transients in patterning processes. Whereas generation of the [Ca2+]i increase was G(alpha2)-independent, only few cells devoid of G(beta) displayed a [Ca2+]i change after stimulation indicating its participation in the regulation of the calcium homeostasis. |
| Databáze: | OpenAIRE |
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