UV-visible scanning spectrophotometry and chemometric analysis as tools for carotenoids analysis in cassava genotypes (Manihot esculenta Crantz)
| Autor: | Rodolfo Moresco, Virgílio G. Uarrota, Aline Pereira, Maíra Tomazzoli, Eduardo da C. Nunes, Luiz Augusto Martins Peruch, Jussara Gazzola, Christopher Costa, Miguel Rocha, Marcelo Maraschin |
|---|---|
| Přispěvatelé: | Universidade do Minho |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Ciências Agrárias::Biotecnologia Agrária e Alimentar
Biotecnologia Agrária e Alimentar [Ciências Agrárias] Manihot Genotype UV-vis Data processing computer science computer systems 010402 general chemistry 01 natural sciences Plant Roots 03 medical and health sciences Cassava genotypes Humans Chemometrics Partial metabolome 030304 developmental biology 2. Zero hunger 0303 health sciences Science & Technology food and beverages Descriptive models General Medicine beta Carotene Carotenoids 0104 chemical sciences RP-HPLC Spectrophotometry Ultraviolet Food Analysis TP248.13-248.65 Biotechnology |
| Zdroj: | Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP Journal of Integrative Bioinformatics, Vol 12, Iss 4, Pp 27-38 (2015) |
| DOI: | 10.2390/biecoll-jib-2015-280 |
| Popis: | In this study, the metabolomics characterization focusing on the carotenoid composition of ten cassava (Manihot esculenta) genotypes cultivated in southern Brazil by UV-visible scanning spectrophotometry and reverse phase-high performance liquid chromatography was performed. Cassava roots rich in -carotene are an important staple food for populations with risk of vitamin A deficiency. Cassava genotypes with high pro-vitamin A activity have been identified as a strategy to reduce the prevalence of deficiency of this vitamin. The data set was used for the construction of a descriptive model by chemometric analysis. The genotypes of yellow-fleshed roots were clustered by the higher concentrations of cis--carotene and lutein. Inversely, cream-fleshed roots genotypes were grouped precisely due to their lower concentrations of these pigments, as samples rich in lycopene (redfleshed) differed among the studied genotypes. The analytical approach (UV-Vis, HPLC, and chemometrics) used showed to be efficient for understanding the chemodiversity of cassava genotypes, allowing to classify them according to important features for human health and nutrition. FAPESC (Fundação de Amparo à Pesquisa e Inovação do Estado de Santa Catarina) and CNPq (Conselho Nacional de Desenvolvimento Cientıfico e Tecnologico) for financial support. The research fellowship from CNPq on behalf of the last author is acknowledged. The work is partially funded by Project PropMine, funded by the agreement between Portuguese FCT and Brazilian CNPq. The authors also thank the FCT Strategic Project of UID/BIO/04469/2013 unit |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|