Sequence-specific photomodification of DNA by an oligonucleotide-phenanthrodihydrodioxin conjugate
| Autor: | Harsch A, Kestutis Bendinskas, Midden Wr, Wilson Rm |
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| Rok vydání: | 1998 |
| Předmět: |
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Stereochemistry Biomedical Engineering Oligonucleotides Pharmaceutical Science Bioengineering Cleavage (embryo) Dioxins chemistry.chemical_compound Photosensitizer Pharmacology Photosensitizing Agents Molecular Structure Oligonucleotide Chemistry Organic Chemistry Quinones Nucleic Acid Hybridization Sequence Analysis DNA Phenanthrenes Cross-Linking Reagents Spectrometry Fluorescence Covalent bond Duplex (building) Spectrophotometry DNA Viral Linker DNA Biotechnology Conjugate Bacteriophage M13 |
| Zdroj: | Bioconjugate chemistry. 9(5) |
| ISSN: | 1043-1802 |
| Popis: | We introduce a new member of a family of photochemically active oligonucleotide conjugates. A Phenanthrodihydrodioxin (PDHD)-based agent was synthesized and covalently linked to a 5'-end of the 9-mer oligonucleotide via a hexamethylene linker. The conjugate hybridized to a complementary 30-nucleotide-long target and efficiently cleaved it in a sequence specific manner. Up to 67% of target was specifically damaged (51% cross-links and 16% direct cleavage). While the photosensitizer alone nonspecifically damaged only Gs in a single-stranded target, its conjugate cross-linked to and damaged also A, T, and C sites in a target in agreement with duplex and triplex formation. |
| Databáze: | OpenAIRE |
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