DARPP-32-like immunoreactivity in AII amacrine cells of rat retina
| Autor: | Leah Haft-Candell, Grant S. Nichols, Gloria J. Partida, Sherwin C. Lee, Andrew T. Ishida |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: |
medicine.medical_specialty
Dopamine and cAMP-Regulated Phosphoprotein 32 Nerve Tissue Proteins Biology Article Retina Amacrine cell Rats Sprague-Dawley chemistry.chemical_compound Dopamine Internal medicine medicine Animals Tyrosine hydroxylase General Neuroscience Retinal Phosphoproteins Adenosine Immunohistochemistry Cell biology Rats medicine.anatomical_structure Endocrinology Amacrine Cells Parvalbumins chemistry biology.protein Female Antibody medicine.drug |
| Popis: | Previous studies demonstrated that the dopamine- and adenosine 3',5'-monophosphate-regulated phosphatase inhibitor known as "DARPP-32" is present in rat, cat, monkey, and human retinas. We have followed up these studies by asking what specific cell subtypes contain DARPP-32. Using a polyclonal antibody directed against a peptide sequence of human DARPP-32, we immunostained adult rat retinas that were either transretinally sectioned or flat mounted and found DARPP-32-like immunoreactivity in some cells of the amacrine cell layer across the entire retinal surface. We report here, based on the shape and spatial distribution of these cells, their staining by an anti-parvalbumin antibody, and their juxtaposition with processes containing tyrosine hydroxylase, that DARPP-32-like immunoreactivity is present in AII amacrine cells of rat retina. These results suggest that the response of AII amacrine cells to dopamine is not mediated as simply as previously supposed. |
| Databáze: | OpenAIRE |
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