Non-targeted and targeted analysis of collagen hydrolysates during the course of digestion and absorption

Autor: Anne J. Kleinnijenhuis, Barbara Vanhoecke, Annet J.H. Maathuis, Yohann Wittrant, Janne Prawitt, Frédérique L. van Holthoon, Fabien Wauquier
Přispěvatelé: Triskelion, Rousselot BVBA, Unité de Nutrition Humaine (UNH), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Rousselot INRA Triskelion, Unité de Nutrition Humaine - Clermont Auvergne (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne (UCA)
Rok vydání: 2019
Předmět:
Zdroj: Analytical and Bioanalytical Chemistry
Analytical and Bioanalytical Chemistry, Springer Verlag, In press, 412 (4), ⟨10.1007/s00216-019-02323-x⟩
Analytical and Bioanalytical Chemistry, In press, 412 (4), ⟨10.1007/s00216-019-02323-x⟩
Analytical and Bioanalytical Chemistry, Springer Verlag, 2019, ⟨10.1007/s00216-019-02323-x⟩
ISSN: 1618-2650
1618-2642
DOI: 10.1007/s00216-019-02323-x
Popis: Protein hydrolysates are an important part of the human diet. Often, they are prepared from milk, soy, or collagen. In the present study, four different collagen hydrolysates were tested, varying in the average molecular weight and the animal source. Three types of samples, the dissolved start products, in vitro generated dialysates (containing the digested components that are potentially available for small intestinal absorption), and human serum collected after product ingestion, were analyzed using LC-MS to compare the state of the hydrolysates before and after absorption, i.e., uptake into the blood. It was found that the composition of the collagen hydrolysates prior to and after ingestion was highly complex and dynamic, which made it challenging to predefine a strategy for a targeted analysis. Therefore, we implemented a new analytical approach to first map hydrolysate data sets by performing non-targeted LC-MS analysis followed by non-targeted and targeted data analysis. It was shown that the insight gained by following such a top down (data) analytical workflow could be crucial for defining a suitable targeted setup and considering data trends beyond the defined targets. After having defined and performed a limited targeted analysis, it was found that, in our experimental setup, Hyp-Gly and especially Pro-Hyp contributed significantly as carrier to the total Hyp increase in blood after ingestion of collagen hydrolysate. Graphical abstract Electronic supplementary material The online version of this article (10.1007/s00216-019-02323-x) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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