Determination of Flurbiprofen and Ibuprofen in Dog Serum with Automated Sample Preparation
| Autor: | L.J. Beaubien, D.J. Sears, Paul D. Rahn, B.G. Snider |
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| Rok vydání: | 1981 |
| Předmět: |
Detection limit
Autoanalysis Chromatography Chemistry Flurbiprofen Extraction (chemistry) Pharmaceutical Science Ibuprofen Hydrogen-Ion Concentration Manual extraction Bioavailability Dogs medicine Animals Sample preparation Propionates Sample extraction Chromatography High Pressure Liquid medicine.drug |
| Zdroj: | Journal of Pharmaceutical Sciences. 70:1347-1349 |
| ISSN: | 0022-3549 |
| DOI: | 10.1002/jps.2600701215 |
| Popis: | Methods for the determination of flurbiprofen and ibuprofen in dog serum were developed using high-performance liquid chromatography and automated serum extraction. Sample extraction was automated by use of cartridges packed with a styrene-divinylbenzene macroreticular resin in a microprocessor-controlled centrifugal system. The average recoveries were 98.9% for flurbiprofen and 94.5% for ibuprofen. The limits of detection were approximately 0.04 microgram/ml for flurbiprofen at 254 nm and 0.5 microgram/ml for ibuprofen at 230 nm. The relative standard deviations for the determination of a laboratory standard between days was 2.4% (20 microgram/ml) for flurbiprofen and 1.7% (13 microgram/ml) for ibuprofen. Peak height ratios were linear with concentrations of 0.04--100 microgram/ml for flurbiprofen and 1.0-50 microgram/ml for ibuprofen. These methods are simple, rapid, sensitive, and specific. The use of an automated sample preparation procedure improved the between-day precision by a factor of two when compared to a manual extraction procedure. These methods were applied to bioavailability studies in dogs. |
| Databáze: | OpenAIRE |
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