Multiparametric labeling optimization and synthesis of68Ga-labeled compounds applying a continuous-flow microfluidic methodology
| Autor: | Szandra Szilágyi, György Trencsényi, László Galuska, Gábor Máté, Jakub Šimeček, Dezso Szikra, István Kertész, Hans-Jürgen Wester |
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| Rok vydání: | 2016 |
| Předmět: |
Fluid Flow and Transfer Processes
Functional evaluation Chromatography 010405 organic chemistry Chemistry Continuous flow Organic Chemistry Microfluidics Nanotechnology Orvostudományok 01 natural sciences High-performance liquid chromatography 030218 nuclear medicine & medical imaging 0104 chemical sciences 03 medical and health sciences 0302 clinical medicine High specific activity Chemistry (miscellaneous) Elméleti orvostudományok |
| Zdroj: | Journal of Flow Chemistry. 6:86-93 |
| ISSN: | 2063-0212 2062-249X |
| Popis: | The synthesis and functional evaluation of a wide variety of radiolabeled chelator–biomolecule conjugates with high specific activity and radiochemical purity are crucial to development of personalized nuclear medicine. An excellent platform technology for achieving this objective involves use of generator-produced positron emission tomography (PET)-radionuclide 68Ga. Currently, applied manual methodology for optimization and development for new labeling techniques offers only slow screening with relatively high precursor consumption. A capillary-based microfluidic synthesis module with online high-performance liquid chromatography (HPLC) was constructed for the optimization of reaction parameters of 68Ga-PET tracers. This approach enables performance of 68Ga-labeling reactions in 10 μL volumes, followed by sample analysis. The high-throughput capacity of the system allows very rapid optimization. The optimal pH and ligand concentration from the experiments were utilized directly to the production of 68Ga... |
| Databáze: | OpenAIRE |
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