Evaluation of third-generation ELISA and a rapid immunochromatographic assay for the detection of norovirus infection in fecal samples from inpatients of a German tertiary care hospital
| Autor: | D. Kaiser, S. Schrempf, G. Geginat |
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| Rok vydání: | 2011 |
| Předmět: |
Adult
Male Microbiology (medical) medicine.medical_specialty Adolescent Enzyme-Linked Immunosorbent Assay Diagnostic tools medicine.disease_cause Polymerase Chain Reaction Sensitivity and Specificity Chromatography Affinity law.invention Feces Young Adult Medical microbiology law Germany medicine Humans Prospective Studies Child Polymerase chain reaction Aged Caliciviridae Infections Aged 80 and over Clinical Laboratory Techniques business.industry Norovirus Infant Newborn Infant General Medicine Middle Aged Tertiary care hospital Virology Hospitals Third generation Gastroenteritis Reverse transcription polymerase chain reaction Infectious Diseases Child Preschool Female business |
| Zdroj: | European Journal of Clinical Microbiology & Infectious Diseases. 31:733-737 |
| ISSN: | 1435-4373 0934-9723 |
| DOI: | 10.1007/s10096-011-1366-z |
| Popis: | The analytical accuracy of the RIDASCREEN Norovirus 3rd Generation ELISA assay and the rapid immunochromatographic RIDAQUICK Norovirus assay were determined in comparison to PCR. In a prospective study 410 consecutive samples were collected from inpatients of a tertiary care hospital in Germany. All samples were tested with the two antigen detection assays, as well as with three different real-time reverse transcription PCR methods as the reference standard. A sample was considered true-positive if at least 2 out of 3 PCR methods yielded a positive signal (137 positive samples, >99% genogroup II). Compared with the PCR-based reference the overall diagnostic sensitivities of the ELISA and the immunochromatographic assay were 77% and 69% and the diagnostic specificities were 96% and 97% respectively. Both assays allow the rapid and economic screening of large numbers of samples and thus are useful diagnostic tools for the detection of suspected norovirus infections. |
| Databáze: | OpenAIRE |
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