The effective bioengineering method of implantation decellularized renal extracellular matrix scaffolds
| Autor: | Kailin Li, Jue Wang, Shengtian Zhao, Yun Luan, Feng Kong, Guanghui Cheng, Yuqiang Liu, Chao Sun, Xiaoshuai Xie, Shuangde Liu, Yong Guan, Denglu Zhang |
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| Rok vydání: | 2015 |
| Předmět: |
Male
medicine.medical_specialty Pathology Scaffold Renal function Bioengineering scaffold Kidney End stage renal disease Extracellular matrix Tissue engineering Animals Medicine Rats Wistar Kidney transplantation Decellularization Tissue Scaffolds urogenital system business.industry recellularization medicine.disease Extracellular Matrix Rats kidney regeneration Surgery medicine.anatomical_structure Oncology tissue engineering decellularization business Research Paper |
| Zdroj: | Oncotarget |
| ISSN: | 1949-2553 |
| DOI: | 10.18632/oncotarget.5304 |
| Popis: | // Yong Guan 1 , Shuangde Liu 2 , Chao Sun 3 , Guanghui Cheng 3 , Feng Kong 3 , Yun Luan 3 , Xiaoshuai Xie 1 , Shengtian Zhao 1 , Denglu Zhang 1 , Jue Wang 3 , Kailin Li 3 , Yuqiang Liu 1 1 The Second Hospital, Shandong University, Department of Urology, Shandong, China 2 The Second Hospital, Shandong University, Department of Kidney Transplantation, Shandong, China 3 The Second Hospital, Shandong University, Department of Central Research Lab, Shandong, China Correspondence to: Shengtian Zhao, e-mail: zhaoshengtian@sdu.edu.cn Keywords: scaffold, tissue engineering, kidney regeneration, decellularization, recellularization Received: March 16, 2015 Accepted: September 11, 2015 Published: September 21, 2015 ABSTRACT End stage renal disease (ESRD) is a progressive loss of kidney function with a high rate of morbidity and mortality. Transplantable organs are hard to come by and hold a high risk of recipient immune rejection. We intended to establish a more effective and faster method to decellularize and recellularize the kidney scaffold for transplant and regeneration. We successfully produced renal scaffolds by decellularizing rat kidneys with 0.5% sodium dodecyl sulfate (SDS), while still preserving the extracellular matrix (ECM) 3D architecture, an intact vascular tree and biochemical components. We recellularized the kidney scaffolds with mouse embryonic stem (ES) cells that then populated and proliferated within the glomerular, vascular, and tubular structures. After in vivo implantation, these recellularized scaffolds were easily reperfused, tolerated blood pressure and produced urine with no blood leakage. Our methods can successfully decellularize and recellularize rat kidneys to produce functional renal ECM scaffolds. These scaffolds maintain their basic components, retain intact vasculature and show promise for kidney regeneration. |
| Databáze: | OpenAIRE |
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