Therapeutic IMC-C225 Antibody Inhibits Breast Cancer Cell Invasiveness via Vav2-Dependent Activation of RhoA GTPase
| Autor: | Poonam R. Molli, Rakesh Kumar, Liana Adam |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
rho GTP-Binding Proteins
Cancer Research VAV2 RHOA Cetuximab Antineoplastic Agents Breast Neoplasms Biology Antibodies Monoclonal Humanized Article Metastasis Epidermal growth factor Cell Line Tumor medicine Humans Neoplasm Invasiveness Epidermal growth factor receptor Neoplasm Metastasis Phosphorylation Proto-Oncogene Proteins c-vav Cytoskeleton Epidermal Growth Factor Antibodies Monoclonal Cancer medicine.disease ErbB Receptors Phenotype Oncology Cancer research biology.protein Female Breast disease Signal transduction rhoA GTP-Binding Protein |
| Zdroj: | Clinical Cancer Research. 14:6161-6170 |
| ISSN: | 1557-3265 1078-0432 |
| Popis: | Purpose: Abnormalities in the expression and signaling pathways downstream of epidermal growth factor receptor (EGFR) contribute to progression, invasion, and maintenance of the malignant phenotype in human cancers. Accordingly, biological agents, such as the EGFR-blocking antibody IMC-C225 have promising anticancer potential and are currently in various stages of clinical development. Because use of IMC-C225 is limited, at present, only for treatment of cancer with high EGFR expression, the goal of the present study was to determine the effect of IMC-C225 on the invasiveness of breast cancer cells with high and low levels of EGFR expression. Experimental Design: The effect of IMC-C225 on invasion was studied using breast cancer cell lines with high and low levels of EGFR expression. Results: The addition of EGF led to progressive stress fiber dissolution. In contrast, cells treated with IMC-C225 showed reduced invasiveness and increased stress-fiber formation. Interestingly, IMC-C225 pretreatment was accompanied by EGFR phosphorylation, as detected using an anti–phosphorylated tyrosine antibody (PY99), which correlated with phosphorylation of Vav2 guanine nucleotide exchange factor and activation of RhoA GTPase irrespective of EGFR level, and Vav2 interacted with EGFR only in IMC-C225–treated cells. The underlying mechanism involved an enhanced interaction between β1 integrins and EGFR upon IMC-C225 treatment. Conclusion: Here, we defined a new mechanism for IMC-C225 that cross-links integrins with EGFR, leading to activation of RhoA and inhibition of breast cancer cell invasion irrespective of the level of EGFR in the cells, thus providing a rationale for using IMC-C225 in the metastatic setting independent of the levels of EGFR. |
| Databáze: | OpenAIRE |
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