Poly C Binding Protein 2 dependent nuclear retention of the utrophin-A mRNA in C2C12 cells

Autor:	Gargi Ghosh, Satyabrata Samui, Santanu Das, Vandana Singh, Doel Pal, Subhanwita Das, Jishu Naskar, Soumya Sinha Roy, Utpal Basu
Rok vydání:	2021
Předmět:	Cell Nucleus Utrophin Recombinant Fusion Proteins RNA-Binding Proteins Cell Biology Brief Communication RNA Transport Cell Line Molecular Imaging Mice Animals RNA Messenger RNA Processing Post-Transcriptional 5' Untranslated Regions Muscle Skeletal Molecular Biology Protein Binding
Zdroj:	RNA Biol
ISSN:	1555-8584 1547-6286
DOI:	10.1080/15476286.2021.2004683
Popis:	Upregulation of utrophin, the autosomal homologue of dystrophin, can compensate dystrophin deficiency in Duchenne Muscular Dystrophy (DMD) although the therapeutic success is yet to be achieved. The present study has identified Poly (C) binding protein 2 (PCBP2) as a post-transcriptional suppresser for the expression of utrophin-A, the muscle-specific utrophin isoform. This study confirms nuclear retention of utrophin-A mRNA in C2C12 cells, which is mediated by PCBP2. Further investigation demonstrates PCBP2-dependent nuclear retention of follistatin mRNA as well. Its involvement in nuclear retention of mRNA sheds light on a novel function of PCBP2 that makes utrophin-A mRNA less available in cytosol. PCBP2, therefore, may be a target to de-repress utrophin-A expression in DMD.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a382745b4c0b45f396d409dc0e3171fd https://doi.org/10.1080/15476286.2021.2004683 Zobrazit plný text záznamu